LOCUS       sequence-142353-       11729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Rodgers JT, Lerin C, Haas W, Gygi SP, Spiegelman BM, Puigserver P
  TITLE     Nutrient control of glucose homeostasis through a complex of 
            PGC-1alpha and SIRT1.
  JOURNAL   Nature 2005 Mar 3;434(7029):113-8.
  PUBMED    15744310
REFERENCE   2  (bases 1 to 11729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2005-03-3"; volume: "434(7029)"; pages: "113-8"
COMMENT     Sequence Label: pAd-Track Flag-SIRT1 H355A
FEATURES             Location/Qualifiers
     source          1..11729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   79..181
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     enhancer        3440..3743
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3744..3947
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4013..4032
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4013..4031
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4032..4056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4071..4093
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4103..4126
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4148..4180
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             4226..6439
                     /codon_start=1
                     /product="mouse NAD+-dependent protein deacetylase 
                     sirtuin-1"
                     /label=SIRT1
                     /note="homolog of budding yeast Sir2"
                     /translation="MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGR
                     SPGEPSAAVAPAAAGCEAASAAAPAALWREAAGAAASAEREAPATAVAGDGDNGSGLRR
                     EPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLLTDGLLTNGFHSCESDDDDRTSHA
                     SSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSE
                     PPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPD
                     LPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNID
                     TLEQVAGIQRILQCAGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIM
                     KPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINR
                     EPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRPQKELVHLS
                     ELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLEVSESSCVEEKPQEVQT
                     SRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGNQYLFV
                     PPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLED
                     DTERPECAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS"
     regulatory      4280..4289
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6650..6771
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        6857..7160
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7161..7364
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      7403..7412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7409..7411,7412..7414,7415..8125)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7409..7411,7412..7414,7415..8125)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7454..7475)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7715..7734)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8062..8083
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8264..8385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_signal     8528..8678
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     repeat_region   8766..8868
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      8906..9286
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8993..9012)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9203..9224
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9334..10128)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(9334..10128)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9445..9464)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10055..10074
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      10960..11548
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11449..11468
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11702..11719
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatgga
       61 tccatgcatg ttaattaaca tcatcaataa tataccttat tttggattga agccaatatg
      121 ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta
      181 ggttttaggg cggagtaact tgtatgtgtt gggaattgta gttttcttaa aatgggaagt
      241 gacgtaacgt gggaaaacgg aagtgacgat ttgaggaagt tgtgggtttt ttggctttcg
      301 tttctgggcg taggttcgcg tgcggttttc tgggtgtttt ttgtggactt taaccgttac
      361 gtcatttttt agtcctatat atactcgctc tgcacttggc ccttttttac actgtgactg
      421 attgagctgg tgccgtgtcg agtggtgttt ttttaatagg ttttcttttt tactggtaag
      481 gctgactgtt atggctgccg ctgtggaagc gctgtatgtt gttctggagc gggagggtgc
      541 tattttgcct aggcaggagg gtttttcagg tgtttatgtg tttttctctc ctattaattt
      601 tgttatacct cctatggggg ctgtaatgtt gtctctacgc ctgcgggtat gtattccccc
      661 gggctatttc ggtcgctttt tagcactgac cgatgtgaat caacctgatg tgtttaccga
      721 gtcttacatt atgactccgg acatgaccga ggagctgtcg gtggtgcttt ttaatcacgg
      781 tgaccagttt ttttacggtc acgccggcat ggccgtagtc cgtcttatgc ttataagggt
      841 tgtttttcct gttgtaagac aggcttctaa tgtttaaatg tttttttgtt attttatttt
      901 gtgtttatgc agaaacccgc agacatgttt gagagaaaaa tggtgtcttt ttctgtggtg
      961 gttccggagc ttacctgcct ttatctgcat gagcatgact acgatgtgct ttcttttttg
     1021 cgcgaggctt tgcctgattt tttgagcagc accttgcatt ttatatcgcc gcccatgcaa
     1081 caagctattg aattcgttta aactccctct caagtctgta tacggggaca cggacagcct
     1141 tttcgtcacc gagcgtggac accggctcat ggaaaccaga ggtaagaaac gcatcaaaaa
     1201 gcatggggga aacctggttt ttgaccccga acggccagag ctcacctggc tcgtggaatg
     1261 cgagaccgtc tgcggggcct gcggcgcgga tgcctactcc ccggaatcgg tatttctcgc
     1321 gcccaagctc tacgccctca aaagtctgca ctgcccctcg tgcggcgcct cctccaaggg
     1381 caagctgcgc gccaagggcc acgccgcgga ggggctggac tatgacacca tggtcaaatg
     1441 ctacctggcc gacgcgcagg gcgaagaccg gcagcgcttc agcaccagca ggaccagcct
     1501 caagcgcacc ctggccagcg cgcagcccgg agcgcacccc ttcaccgtga cccagactac
     1561 gctgacgagg accctgcgcc cgtggaaaga catgaccctg gcccgtctgg acgagcaccg
     1621 actactgccg tacagcgaaa gccgccccaa cccgcgaaac gaggagatat gctggatcga
     1681 gatgccgtag agcaggtgac cgagctgtgg gaccgcctgg aactgcttgg tcaaacgctc
     1741 aaaagcatgc ctacggcgga cggtctcaaa ccgttgaaaa actttgcttc cttgcaagaa
     1801 ctgctatcgc tgggcggcga gcgccttctg gcggatttgg tcagggaaaa catgcgagtc
     1861 agggacatgc ttaacgaagt ggcccccctg ctcagggatg acggcagctg cagctctctt
     1921 aactaccagt tgcagccggt aataggtgtg atttacgggc ccaccggctg cggtaagtcg
     1981 cagctgctca ggaacctgct ttcttcccag ctgatctccc ctaccccgga aaccgttttc
     2041 ttcatcgccc cgcaggtaga catgatcccc ccatctgaac tcaaagcgtg ggaaatgcaa
     2101 atctgtgagg gtaactacgc ccctgggccg gatggaacca ttataccgca gtctggcacc
     2161 ctccgcccgc gctttgtaaa aatggcctat gacgatctca tcctggaaca caactatgac
     2221 gttagtgatc ccagaaatat cttcgcccag gccgccgccc gtgggcccat tgccatcatt
     2281 atggacgaat gcatggaaaa tcttggaggt cacaagggcg tctccaagtt cttccacgca
     2341 tttccttcta agctacatga caaatttccc aagtgcaccg gatacactgt gctggtggtt
     2401 ctgcacaaca tgaatccccg gagggatatg gctgggaaca tagccaacct aaaaatacag
     2461 tccaagatgc atctcatatc cccacgtatg cacccatccc agcttaaccg ctttgtaaac
     2521 acttacacca agggcctgcc cctggcaatc agcttgctac tgaaagacat ttttaggcac
     2581 cacgcccagc gctcctgcta cgactggatc atctacaaca ccaccccgca gcatgaagct
     2641 ctgcagtggt gctacctcca ccccagagac gggcttatgc ccatgtatct gaacatccag
     2701 agtcaccttt accacgtcct ggaaaaaata cacaggaccc tcaacgaccg agaccgctgg
     2761 tcccgggcct accgcgcgcg caaaacccct aaataaagac agcaagacac ttgcttgatc
     2821 caaatccaaa cagagtctgg ttttttattt atgttttaaa ccgcattggg aggggaggaa
     2881 gccttcaggg cagaaacctg ctggcgcaga tccaacagct gctgagaaac gacattaagt
     2941 tcccgggtca aagaatccaa ttgtgccaaa agagccgtca acttgtcatc gcgggcggat
     3001 gaacgggaag ctgcactgct tgcaagcggg ctcaggaaag caaagtcagt cacaatcccg
     3061 cgggcggtgg ctgcagcggc tgaagcggcg gcggaggctg cagtctccaa cggcgttcca
     3121 gacacggtct cgtaggtcaa ggtagtagag tttgcgggca ggacggggcg accatcaatg
     3181 ctggagccca tcacattctg acgcaccccg gcccatgggg gcatgcgcgt tgtcaaatat
     3241 gagctcacaa tgcttccatc aaacgagttg gtgctcatgg cggcggcggc tgctgcaaaa
     3301 cagatacaaa actacataag acccccacct tatatattct ttcccaccct taaccacgcc
     3361 cagatcctct agcagtgata aacgtctaat agtaatcaat tacggggtca ttagttcata
     3421 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     3481 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     3541 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     3601 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     3661 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     3721 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     3781 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     3841 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     3901 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
     3961 gtcagatccg ctagagatct ggtaccgagc tcggatctcg atcccgcgaa attaatacga
     4021 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
     4081 tttaagaagg agatatacca tggactacaa agacgatgac gataaagcaa gaagagcatc
     4141 tgtgcatatg gctagcatga ctggtggaca gcaaatgggt cgcggatccg aattcgagct
     4201 ccgtcgacag agaggcagtt ggaagatggc ggacgaggtg gcgctcgccc ttcaggccgc
     4261 cggctcccct tccgcggcgg ccgccatgga ggccgcgtcg cagccggcgg acgagccgct
     4321 ccgcaagagg ccccgccgag acgggcctgg cctcgggcgc agcccgggcg agccgagcgc
     4381 agcagtggcg ccggcggccg cggggtgtga ggcggcgagc gccgcggccc cggcggcgct
     4441 gtggcgggag gcggcagggg cggcggcgag cgcggagcgg gaggccccgg cgacggccgt
     4501 ggccggggac ggagacaatg ggtccggcct gcggcgggag ccgagggcgg ccgacgactt
     4561 cgacgacgac gagggcgagg aggaggacga ggcggcggcg gcagcggcgg cggcagcgat
     4621 cggctaccga gacaacctcc tgttgaccga tggactcctc actaatggct ttcattcctg
     4681 tgaaagtgat gacgatgaca gaacgtcaca cgccagctct agtgactgga ctccgcggcc
     4741 gcggataggt ccatatactt ttgttcagca acatctcatg attggcaccg atcctcgaac
     4801 aattcttaaa gatttattac cagaaacaat tcctccacct gagctggatg atatgacgct
     4861 gtggcagatt gttattaata tcctttcaga accaccaaag cggaaaaaaa gaaaagatat
     4921 caatacaatt gaagatgctg tgaagttact gcaggagtgt aaaaagataa tagttctgac
     4981 tggagctggg gtttctgtct cctgtgggat tcctgacttc agatcaagag acggtatcta
     5041 tgctcgcctt gcggtggact tcccagacct cccagaccct caagccatgt ttgatattga
     5101 gtattttaga aaagacccaa gaccattctt caagtttgca aaggaaatat atcccggaca
     5161 gttccagccg tctctgtgtc acaaattcat agctttgtca gataaggaag gaaaactact
     5221 tcgaaattat actcaaaata tagatacctt ggagcaggtt gcaggaatcc aaaggatcct
     5281 tcagtgtgct ggttcctttg caacagcatc ttgcctgatt tgtaagtaca aagttgattg
     5341 tgaagctgtt cgtggagaca tttttaatca ggtagttcct cggtgcccta ggtgcccagc
     5401 tgatgagcca cttgccatca tgaagccaga gattgtcttc tttggtgaaa acttaccaga
     5461 acagtttcat agagccatga agtatgacaa agatgaagtt gacctcctca ttgttattgg
     5521 atcttctctg aaagtgagac cagtagcact aattccaagt tctatacccc atgaagtgcc
     5581 tcaaatatta ataaataggg aacctttgcc tcatctacat tttgatgtag agctccttgg
     5641 agactgcgat gttataatta atgagttgtg tcataggcta ggtggtgaat atgccaaact
     5701 ttgttgtaac cctgtaaagc tttcagaaat tactgaaaaa cctccacgcc cacaaaagga
     5761 attggttcat ttatcagagt tgccaccaac acctcttcat atttcggaag actcaagttc
     5821 acctgaaaga actgtaccac aagactcttc tgtgattgct acacttgtag accaagcaac
     5881 aaacaacaat gttaatgatt tagaagtatc tgaatcaagt tgtgtggaag aaaaaccaca
     5941 agaagtacag actagtagga atgttgagaa cattaatgtg gaaaatccag attttaaggc
     6001 tgttggttcc agtactgcag acaaaaatga aagaacttca gttgcagaaa cagtgagaaa
     6061 atgctggcct aatagacttg caaaggagca gattagtaag cggcttgagg gtaatcaata
     6121 cctgtttgta ccaccaaatc gttacatatt ccacggtgct gaggtatact cagactctga
     6181 agatgacgtc ttgtcctcta gttcctgtgg cagtaacagt gacagtggca catgccagag
     6241 tccaagttta gaagaaccct tggaagatga aagtgaaatt gaagaattct acaatggctt
     6301 ggaagatgat acggagaggc ccgaatgtgc tggaggatct ggatttggag ctgatggagg
     6361 ggatcaagag gttgttaatg aagctatagc tacaagacag gaattgacag atgtaaacta
     6421 tccatcagac aaatcataac actattgaag ctgtccggat tcaggaattg gtcgacaagc
     6481 ttgcggccgc actcgagcct aagcttctag ataagatatc cgatccaccg gatctagata
     6541 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     6601 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     6661 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6721 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcggagg
     6781 tttatcgacg atctgctagt gattaatagt aatcaattac ggggtcatta gttcatagcc
     6841 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     6901 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     6961 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7021 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     7081 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     7141 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     7201 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     7261 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     7321 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
     7381 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     7441 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     7501 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     7561 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     7621 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     7681 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     7741 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     7801 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     7861 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     7921 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     7981 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     8041 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     8101 tctcggcatg gacgagctgt acaagtccgg actcagatcc accggatcta gataactgat
     8161 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     8221 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     8281 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8341 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg atcaagctag
     8401 cttgctagac tcgactgact ataataataa aacgccaact ttgacccgga acgcggaaaa
     8461 cacctgagaa aaacacctgg gcgagtctcc acgtaaacgg tcaaagtccc cgcggcccta
     8521 gacaaatatt acgcgctatg agtaacacaa aattattcag atttcacttc ctcttattca
     8581 gttttcccgc gaaaatggcc aaatcttact cggttacgcc caaatttact acaacatccg
     8641 cctaaaaccg cgcgaaaatt gtcacttcct gtgtacaccg gcgcacacca aaaacgtcac
     8701 ttttgccaca tccgtcgctt acatgtgttc cgccacactt gcaacatcac acttccgcca
     8761 cactactacg tcacccgccc cgttcccacg ccccgcgcca cgtcacaaac tccaccccct
     8821 cattatcata ttggcttcaa tccaaaataa ggtatattat tgatgatgtt aattaagaat
     8881 taattcgatc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     8941 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     9001 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     9061 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9121 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9181 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9241 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     9301 aatgagctga tttaacaaaa attttaacaa aattcagaag aactcgtcaa gaaggcgata
     9361 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     9421 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     9481 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     9541 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgct
     9601 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     9661 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     9721 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     9781 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     9841 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     9901 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag
     9961 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
    10021 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
    10081 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
    10141 tcatcctgtc tcttgatcag agcttgatcc cctgcgccat cagatccttg gcggcaagaa
    10201 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
    10261 ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact
    10321 gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
    10381 gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtga aaaggatcta
    10441 ggtgaagatc ctttttgata atctcatggc tgcagcaatg gcaacaacgt tgcgcaaact
    10501 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    10561 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    10621 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    10681 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    10741 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    10801 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    10861 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    10921 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    10981 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    11041 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    11101 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    11161 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    11221 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    11281 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    11341 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    11401 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    11461 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    11521 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    11581 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    11641 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    11701 cagcgagtca gtgagcgagg aagcggaag
//
