LOCUS       sequence-142359-        7373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7373)
  TITLE     wt HA-dynamin 1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: wt dynamin 2 pEGFP
FEATURES             Location/Qualifiers
     source          1..7373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1364..1375
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      3320..3329
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3326..3328,3329..3331,3332..4045)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3326..3328,3329..3331,3332..4042)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3371..3392)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3632..3651)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3979..4000
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4168..4289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4205..4224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4259..4278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4296..4751)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4433..4454)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4645..4664
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4778..4882
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4884..5241
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4884..5075
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4907..4927)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5092..5227
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5276..6070
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5276..6070
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5330..5349)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5940..5959
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6258..6277)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6302..6349
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6678..7266
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7167..7186
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      601 agcgctaccg gactcagatc tcgagctcaa gcttggcacc atgggcaacc gcgggatgga
      661 agagctcatc ccgctggtta acaagctgca ggacgccttc agttccatcg gtcagagctg
      721 ccacctggac ctgccgcaga tcgccgtggt gggcggccag agcgccggca agagttcggt
      781 gctcgagaac ttcgtgggcc gggacttcct tccacgagga tcaggaattg tcacccggag
      841 gcctctcatt ctgcagctca tcttttccaa aacagaatat gcggagtttt tgcactgcaa
      901 gtccaaaaag tttacagact ttgatgaagt ccggcaggag atcgaagcag agactgaccg
      961 ggtcacaggc accaacaaag gcatctcccc ggtgcccatc aaccttcggg tctattcacc
     1021 acacgtgttg aacttgaccc tcatcgacct cccaggcatc actaaggtgc cagtggggga
     1081 ccagccacca gacatcgagt accagatcaa ggacatgatc ctgcagttta tcagccggga
     1141 gagcagcctc attcttgccg tcacacctgc caacatggac ttggccaact cggatgccct
     1201 caagctggcc aaggaggtgg acccccaagg cctgcggacc attggtgtca tcaccaagct
     1261 agacctgatg gatgaaggca cagatgccag ggatgtcctg gagaacaagc tcctgccctt
     1321 gagaagaggc tacatcggcg tggttaaccg cagccagaaa gacatcgagg gcagaaagga
     1381 catccgggca gctctggcag ctgagaggaa attcttcctc tcccacccag cctaccggca
     1441 catggctgac cgcatgggta ccccacactt gcagaaaaca ctgaaccagc aactgaccaa
     1501 ccacatccgg gagtcactgc caaccctgcg cagcaagctg cagagccaac tgctgtccct
     1561 ggagaaggaa gtggaagagt acaagaattt ccggcctgac gaccccacac gcaagaccaa
     1621 agccctgctg cagatggtcc agcagtttgg agtggacttt gagaagcgaa tcgaaggctc
     1681 tggagatcaa gtcgacaccc tggagctgtc tgggggcgcc cgcatcaatc gcatcttcca
     1741 cgagcgcttt ccctttgagc tggtgaagat ggagtttgac gagaaagacc tccgaagaga
     1801 gatcagctac gccattaaga acatccacgg agtcaggacg gggctcttca cccccggcat
     1861 ggcctttgaa gccatcgtga aaaaacaact tgtaaagctc aaagagccga gtttgaagtg
     1921 tgttgacctg gtggtgtcgg agctagccac ggtcattaaa aagtgtgctg agaagctcag
     1981 ttcctacccc cgccttcgag aggagaccga acgcattgtc accacctaca tcagggagcg
     2041 agaagggaga accaaggacc agatccttct tctgattgac attgagcagt cctacatcaa
     2101 cacaaaccat gaagacttca ttggatttgc caatgcccag cagaggagca cgcagctgaa
     2161 caagaagagg gccataccta accaggggga gatcttggtg atccgcaggg gctggttgac
     2221 catcaacaac atcagcttga tgaaaggcgg ttccaaggag tactggttcg tgctgactgc
     2281 tgagtcgttg tcttggtaca aggatgaaga ggaaaaagaa aagaagtaca tgctgccact
     2341 agacaacctc aaaatacggg atgtggagaa gggcttcatg tccaacaagc atgtgtttgc
     2401 catcttcaac acagagcaga ggaacgtcta caaggacctg cgacagatcg aactggcttg
     2461 tgactcccag gaagatgtgg acagctggaa ggcttcgttc ctgcgggctg gggtctaccc
     2521 agagaaggac caggcagaga acgaggatgg agcacaagag aacaccttct ccatggaccc
     2581 gcagctggag cggcaggtgg agaccatccg caacttggtg gactcctatg cggccatcat
     2641 caacaagtcc atccgggacc tcatgccaaa gaccatcatg cacctcatga tcaacaacac
     2701 aaaggccttc atccgccatg agctgctagc ctacctgtac tcatcagcag accagagcag
     2761 cctcatggag gagtcagctg agcaggctca gcggcgggac gatatgctgc gcatgtacca
     2821 tgcactcaag gaagcgctca acatcattgg ggacatcagc accagcaccg tgtccacgcc
     2881 tgtgccccca cctgtcgatg atacttggct ccggaacacc agcagccaca gccccactcc
     2941 acagcgcaga cctgtgtcca gtgtgcaccc gccgggccgc ccacccgcag tgcggggccc
     3001 cacaccgggg cctcccctca ttcctatgcc tgtgggggcc acatcctcct tctcggcacc
     3061 ccccatccca tcccggcctg gaccacagaa tgtgtttgcc aacaatgacc ccttctctgc
     3121 gccacctcag ataccgtctc ggccagcacg gattccccct gggatccctc caggagtgcc
     3181 cagcagaaga gcgcctgctg cgcccagccg gcccaccatt atccgcccag ccgagccgtc
     3241 cctgctcgac gctgcagggg acagtgttct ggggggaatt ctgcagtcga cggtaccgcg
     3301 ggcccgggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     3361 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3421 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3481 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     3541 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3601 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3661 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3721 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3781 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     3841 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3901 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     3961 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     4021 cggcatggac gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc
     4081 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     4141 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4201 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4261 ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt
     4321 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     4381 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     4441 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     4501 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     4561 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     4621 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     4681 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     4741 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4801 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4861 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     4921 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4981 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5041 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5101 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5161 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5221 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     5281 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     5341 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     5401 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     5461 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     5521 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     5581 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     5641 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     5701 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     5761 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     5821 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     5881 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     5941 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     6001 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     6061 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     6121 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6181 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6241 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     6301 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     6361 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     6421 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     6481 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     6541 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6601 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6661 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6721 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6781 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6841 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6901 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6961 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7021 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7081 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7141 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7201 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7261 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7321 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgc
//
