LOCUS       sequence-142373-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For cloning and constitutive expression of sgRNAs in E.coli. New 
            sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned 
            into BsaI sites. Kanamycin resistant with pSC101 origin of rep..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Standage-Beier K, Zhang Q, Wang X
  TITLE     Targeted Large-Scale Deletion of Bacterial Genomes Using 
            CRISPR-Nickases.
  JOURNAL   ACS Synth Biol. 2015 Nov 20;4(11):1217-25. doi: 
            10.1021/acssynbio.5b00132. Epub 2015 Oct 25.
  PUBMED    26451892
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00132"; journalName: "ACS Synth Biol"; date: 
            "2015-11-20- 20"; volume: "4"; issue: "11"; pages: "1217-25"
COMMENT     Sequence Label: pSG4K5
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(46..80)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     terminator      134..163
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      319..413
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 cttgctattt ctagctctaa aacagaagag cctgtcgctc ttctagctag cattatacct
       61 aggactgagc tagctgtcaa cgaattcgag tcactaaggg ctaactaact aattacgtag
      121 caatcaactc actggctcac cttcacgggt gggcctttct tcggcacggg caaattgctg
      181 aatattcctt ttcttagacg tcaggtggca aacgcgaagt aatcttttcg gttttaaaga
      241 aaaagggcag ggtggtgaca ccttgcccgt ttttttgccg gagctagagc tgtcagacca
      301 agtttacgag ctcgcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
      361 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagca
      421 ctagggacag taagacgggt aagcctgttg atgataccgc tgccttactg ggtgcattag
      481 ccagtctgaa tgacctgtca cgggataatc cgaagtggtc agactggaaa atcagagggc
      541 aggaactgct gaacagcaaa aagtcagata gcaccacata gcagacccgc cataaaacgc
      601 cctgagaagc ccgtgacggg cttttcttgt attatgggta gtttccttgc atgaatccat
      661 aaaaggcgcc tgtagtgcca tttaccccca ttcactgcca gagccgtgag cgcagcgaac
      721 tgaatgtcac gaaaaagaca gcgactcagg tgcctgatgg tcggagacaa aaggaatatt
      781 cagcgatttg cccgagcttg cgagggtgct acttaagcct ttagggtttt aaggtctgtt
      841 ttgtagagga gcaaacagcg tttgcgacat ccttttgtaa tactgcggaa ctgactaaag
      901 tagtgagtta tacacagggc tgggatctat tctttttatc tttttt
//
