LOCUS       sequence-142378-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3XFLAG::GR LBD::eGFP from the hsp16.48 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Monsalve GC, Yamamoto KR, Ward JD
  TITLE     A New Tool for Inducible Gene Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2019 Feb;211(2):419-430. doi: 10.1534/genetics.118.301705.
            Epub 2018 Nov 30.
  PUBMED    30504365
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.118.301705"; journalName: "Genetics"; date: 
            "2019-02"; volume: "211"; issue: "2"; pages: "419-430"
COMMENT     Sequence Label: pJW1241
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(949..969)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(976..999)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggagaacttg tgtccgttga cgtctccgtc gagctcgacg aggattggga cgactccggt
       61 gaagagctcc tctcccttgg acatctgcag cttttgatga aacagaagtt ttttgatatt
      121 tccatttgaa tattttggta tctgattggt gatgatttca gctaacatct cggggaattc
      181 aatactcatg gtcttatcca aaaatgtttg gaagcaatag ttaaggagat tttcaaccac
      241 ttcatgcata gaatccaaga gttttgtcag ttgataaaac cgctgccagt tctggctgga
      301 gtttccttcc ctcttgacaa tggcttttcc tagctctttg atgtaggtca ttctaatttc
      361 atcaaatagc tcttggctct tcagaccgtc cttaggaact gaagagagaa gcagtaaggt
      421 tttcatacag agatactctt cataagatac ctgaagcctg tgtaactcag aggaaacata
      481 cagcatgtgt ttacattggt cgtacatgca gggtagagtc attctctgct cattaataat
      541 cagatcagga gcaaaacaca gcaggtttgc acttgattgt ctatatgatc tccaccccag
      601 agcaaatgcc ataagaaaca tccaggagta ctgcagtagg gtcatttggt catccaggtg
      661 taagttcctg aaacctggta ttgcctttgc ccatttcact gctgcaatca cttgccgccc
      721 tcctaacatg ttgagcgtag tcatgatcct ccaagttgag tctggaacag agctatcata
      781 tcctgcatat aacacttcag gttcaataac ctccaacagt gacaccaggg taggggtgag
      841 ttgtggtaac gttgcaggaa ctattgtttt gttaccagga ttttcagagg tttcttgtga
      901 gactcctgta gtggcctgct gaatggcggc tgggactccg tggattccga ccttacgctt
      961 cttctttggg gccatcttgt cgtcgtcgtc cttgtagtc
//
