LOCUS       sequence-142385-        6472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Osteopontin from transcript tv2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6472)
  TITLE     CAG.Luc.puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBABE.is2-mOpn
FEATURES             Location/Qualifiers
     source          1..6472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             98..567
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    632..831
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1024..1440
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1408..1424
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2392..2412)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2397..2726
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2577..2712
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2800..3825
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2800..3825
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             4620..5089
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(5574..6365,6366..6434))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6197..6216
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6435..6472,1..67))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
       61 ttccgcgcac atttccccga aaagtgccac ctgcagcctg aatatgggcc aaacaggata
      121 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
      181 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
      241 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
      301 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
      361 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
      421 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
      481 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      541 tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg
      601 cccagggacc accgacccac caccgggagg caagctggcc agcaacttat ctgtgtctgt
      661 ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact
      721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
      901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
      961 tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
     1021 tagggccaga ctgttaccac tcccttaagt ttgaccttag atcactggaa agatgtcgag
     1081 cggctcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
     1141 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
     1201 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
     1261 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
     1321 gtacacccta agcctccgcc tcctcttctt ccatccgcgc cgtctctccc ccttgaacct
     1381 cctctttcga ccccgcctca atcctccctt tatccagccc tcactccttc tctaggcgcc
     1441 gggatccatg aggctgcagt tctcctggct gaattctgag ggactaacta cgaccatgag
     1501 attggcagtg atttgctttt gcctgtttgg cattgcctcc tccctcccgg tgaaagtgac
     1561 tgattctggc agctcagagg agaagcttta cagcctgcac ccagatccta tagccacatg
     1621 gctggtgcct gacccatctc agaagcagaa tctccttgcg ccacagaatg ctgtgtcctc
     1681 tgaagaaaag gatgacttta agcaagaaac tcttccaagc aattccaatg aaagccatga
     1741 ccacatggac gacgatgatg acgatgatga tgacgatgga gaccatgcag agagcgagga
     1801 ttctgtggac tcggatgaat ctgacgaatc tcaccattcg gatgagtctg atgagaccgt
     1861 cactgctagt acacaagcag acactttcac tccaatcgtc cctacagtcg atgtccccaa
     1921 cggccgaggt gatagcttgg cttatggctt gagatcaaag tctaggagtt tccaggtttc
     1981 tgatgaacag tatcctgatg ccacagatga ggacctcacc tctcacatga agagcggtga
     2041 gtctaaggag tccctcgatg tcatccctgt tgcccagctt ctgagcatgc cctctgatca
     2101 ggacaacaac ggaaagggca gccatgagtc aagtcagctg gatgaaccaa gtctggaaac
     2161 acacagactt gagcattcca aagagagcca ggagagtgcc gatcagtcgg atgtgatcga
     2221 tagtcaagca agttccaaag ccagcctgga acatcagagc cacaagtttc acagccacaa
     2281 ggacaagcta gtcctagacc ctaagagtaa ggaagatgat aggtatctga aattccgaat
     2341 ttctcatgaa ttagagagtt catcttctga ggtcaactaa gtcgaccctg tggaatgtgt
     2401 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2461 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     2521 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     2581 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     2641 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     2701 tttttggagg cctaggcttt tgcaaaaagc ttgccagctg gggcgccctc tggtaaggtt
     2761 gggaagccct gcagatctga tcccgggggg caatgagata tgaaaaagcc tgaactcacc
     2821 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     2881 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     2941 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     3001 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaatttag cgagagcctg
     3061 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     3121 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     3181 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     3241 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     3301 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     3361 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     3421 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     3481 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     3541 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     3601 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     3661 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     3721 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     3781 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagagtag atgccgaccg
     3841 ggatctatcg atgcggcggg tgtggtggtt acgcgtaatc tgctgcttgc aaacaaaaaa
     3901 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3961 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4021 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4081 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4141 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4201 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4261 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4321 gcgcacgagg gagcttccag gggaaacgcc tggtatctta tagtcctgtc gggtttcgcc
     4381 acctctgact tgagcgtcga ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4441 cgccagcaac gcatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     4501 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     4561 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc
     4621 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     4681 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     4741 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
     4801 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     4861 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     4921 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     4981 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     5041 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg
     5101 tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca tagtgcggcc
     5161 gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5221 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5281 tcgtgtagat aactacgata cgggagggct taccatctgg ccccccaagg cggtaatacg
     5341 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     5401 ggccaggaac cgtaaaaagg ccgcgttacg ctcagtggaa cgaaaactca cgttaaggga
     5461 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5521 gtttgcggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5581 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5641 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5701 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5761 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5821 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5881 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5941 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6001 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     6061 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     6121 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     6181 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6241 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6301 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6361 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6421 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gg
//
