LOCUS       sequence-142388-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3XFLAG::GR LBD::DAF-16A from the hsp16.48 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Monsalve GC, Yamamoto KR, Ward JD
  TITLE     A New Tool for Inducible Gene Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2019 Feb;211(2):419-430. doi: 10.1534/genetics.118.301705.
            Epub 2018 Nov 30.
  PUBMED    30504365
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.118.301705"; journalName: "Genetics"; date: 
            "2019-02"; volume: "211"; issue: "2"; pages: "419-430"
COMMENT     Sequence Label: pGM59
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        52..356
                     /label=hsp16-48 promoter
                     /note="promoter for the C. elegans heat shock protein 16-48
                     gene (Russnak and Candido, 1985)"
     CDS             370..435
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             442..462
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aagtgacatg attgtagttt gaagatttca caattagagt gaatgttgtt tggttcggtt
       61 ttgtcactgt atttatactc atttccacct ttttctagaa cattcgagct gcttcttaca
      121 aaaggaggcg actcacattc agaacattga gaaatagtgt gcgactgaag aaacccagat
      181 actttttcaa tctgcgtctc tttgcaccta tggggtgtat tttgaaatga atgcatctag
      241 gaccttctag aacattctaa acggctgcag gatacgggta tataagccaa tcgtgttcag
      301 aggaaaccaa tacactttgt tcaagtgctt actgttcatt ctctaaactt caagaactag
      361 aaaaaaatgg actacaagga ccacgacgga gactacaagg accacgacat cgactacaag
      421 gacgacgacg acaagatggc cccaaagaag aagcgtaagg tcggaatcca cggagtccca
      481 gccgccattc agcaggccac tacaggagtc tcacaagaaa cctctgaaaa tcctggtaac
      541 aaaacaatag tacctgcaac gttaccacaa ctcaccccta ccctggtgtc actgttggag
      601 gttattgaac ctgaagtgtt atatgcagga tatgatagct ctgttccaga ctcaacttgg
      661 aggatcatga ctacgctcaa catgttagga gggcggcaag tgattgcagc agtgaaatgg
      721 gcaaaggcaa taccaggttt caggaactta cacctggatg accaaatgac cctactgcag
      781 tactcctgga tgtttcttat ggcatttgct ctggggtgga gatcatatag acaatcaagt
      841 gcaa
//
