LOCUS       sequence-142390-       10646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Darzacq X, Shav-Tal Y, de Turris V, Brody Y, Shenoy SM, Phair RD, 
            Singer RH
  TITLE     In vivo dynamics of RNA polymerase II transcription.
  JOURNAL   Nat Struct Mol Biol. 2007 Sep;14(9):796-806. Epub 2007 Aug 5.
  PUBMED    17676063
REFERENCE   2  (bases 1 to 10646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol."; date: "2007-09"; volume: "14(9)"; pages: "796-806. Epub 
            2007 Aug 5"
COMMENT     Sequence Label: pYFP-RPB1aAmr
FEATURES             Location/Qualifiers
     source          1..10646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        60..363
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        364..567
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     518..538
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      606..615
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(612..614,615..617,618..1328)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(918..937)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1265..1286
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1329..1394
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1946..1955
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2349..2358
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7434..7555
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7471..7490)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7525..7544
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7562..8017)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7699..7720)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7911..7930
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8044..8148
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8150..8507
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8150..8341
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8173..8193)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8358..8493
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8542..9336
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8542..9336
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8596..8615)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9206..9225
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9524..9543)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9568..9615
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9944..10532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10433..10452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
       61 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      121 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      181 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      241 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      301 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      361 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      421 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      481 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      541 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
      601 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      661 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      721 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      781 cctggcccac cctcgtgacc accttcggct acggcctgca gtgcttcgcc cgctaccccg
      841 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      901 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      961 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1021 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     1081 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     1141 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1201 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     1261 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1321 tgtacaagtc cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg
     1381 cgggcccggg atccaccgga tctagcatgc acgggggtgg ccccccctcg ggggacagcg
     1441 catgcccgct gcgcaccatc aagagagtcc agttcggagt cctgagtccg gatgaactga
     1501 agcgaatgtc tgtgacggag ggtggcatca aatacccaga gacgactgag ggaggccgcc
     1561 ccaagcttgg ggggctgatg gacccgaggc agggggtgat tgagcggact ggccgctgcc
     1621 aaacatgtgc aggaaacatg acagagtgtc ctggccactt tggccacatt gaactggcca
     1681 agcctgtgtt tcacgtgggc ttcctggtga agacaatgaa agttttgcgc tgtgtctgct
     1741 tcttctgctc caaactgctt gtggactcta acaacccaaa gatcaaggat atcctggcta
     1801 agtccaaggg acagcccaag aagcggctca cacatgtcta cgacctttgc aagggcaaaa
     1861 acatatgcga gggtggggag gagatggaca acaagttcgg tgtggaacaa cctgagggtg
     1921 acgaggatct gaccaaagaa aagggccatg gtggctgtgg gcggtaccag cccaggatcc
     1981 ggcgttctgg cctagagctg tatgcggaat ggaagcacgt taatgaggac tctcaggaga
     2041 agaagatcct gctgagtcca gagcgagtgc atgagatctt caaacgcatc tcagatgagg
     2101 agtgttttgt gctgggcatg gagccccgct atgcacggcc agagtggatg attgtcacag
     2161 tgctgcctgt gcccccgctc tccgtgcggc ctgctgttgt gatgcagggc tctgcccgta
     2221 accaggatga cctgactcac aaactggctg acatcgtgaa gatcaacaat cagctgcggc
     2281 gcaatgagca gaacggcgca gcggcccatg tcattgcaga ggatgtgaag ctcctccagt
     2341 tccatgtggc caccatggtg gacaatgagc tgcctggctt gccccgtgcc atgcagaagt
     2401 ctgggcgtcc cctcaagtcc ctgaagcagc ggttgaaggg caaggaaggc cgggtgcgag
     2461 ggaacctgat gggcaaaaga gtggacttct cggcccgtac tgtcatcacc cccgacccca
     2521 acctctccat tgaccaggtt ggcgtgcccc gctccattgc tgccaacatg acctttgcgg
     2581 agattgtcac ccccttcaac attgacagac ttcaagaact agtgcgcagg gggaacagcc
     2641 agtacccagg cgccaagtac atcatccgag acaatggtga tcgcattgac ttgcgtttcc
     2701 accccaagcc cagtgacctt cacctgcaga ccggctataa ggtggaacgg cacatgtgtg
     2761 atggggacat tgttatcttc aaccggcagc caactctgca caaaatgtcc atgatggggc
     2821 atcgggtccg cattctccca tggtctacct ttcgcttgaa tcttagcgtg acaactccgt
     2881 acaatgcaga ctttgacggg gatgagatga acttgcacct gccacagtct ctggagacgc
     2941 gagcagagat ccaggagctg gccatggttc ctcgcatgat tgtcaccccc cagagcaatc
     3001 ggcctgtcat gggtattgtg caggacacac tcacagcagt gcgcaaattc accaagagag
     3061 acgtcttcct ggagcggggt gaagtgatga acctcctgat gttcctgtcg acgtgggatg
     3121 ggaaggtccc acagccggcc atcctaaagc cccggcccct gtggacaggc aagcaaatct
     3181 tctccctcat catacctggt cacatcaatt gtatccgtac ccacagcacc catcccgatg
     3241 atgaagacag tggcccttac aagcacatct ctcctgggga caccaaggtg gtggtggaga
     3301 atggggagct gatcatgggc atcctgtgta agaagtctct gggcacgtca gctggctccc
     3361 tggtccacat ctcctaccta gagatgggtc atgacatcac tcgcctcttc tactccaaca
     3421 ttcagactgt cattaacaac tggctcctca tcgagggtca tactattggc attggggact
     3481 ccattgctga ttctaagact taccaggaca ttcagaacac tattaagaag gccaagcagg
     3541 acgtaataga ggtcatcgag aaggcacaca acaatgagct ggagcccacc ccagggaaca
     3601 ctctgcggca gacgtttgag aatcaggtga accgcattct taacgatgcc cgagacaaga
     3661 ctggctcctc tgctcagaaa tccctgtctg aatacaacaa cttcaagtct atggtcgtgt
     3721 ccggagctaa aggttccaag attaacatct cccaggtcat tgctgtcgtt ggacagcagg
     3781 acgtcgaggg caagcggatt ccatttggct tcaagcaccg gactctgcct cacttcatca
     3841 aggatgacta cgggcctgag agccgtggct ttgtggagaa ctcctaccta gccggcctca
     3901 cacccactga gttctttttc cacgccatgg ggggtcgtga ggggctcatt gacacggctg
     3961 tcaagactgc tgagactgga tacatccagc ggcggctgat caagtccatg gagtcagtga
     4021 tggtgaagta cgacgcgact gtgcggaact ccatcaacca ggtggtgcag ctgcgctacg
     4081 gcgaagacgg cctggcaggc gagagcgttg agttccagaa cctggctacg cttaagcctt
     4141 ccaacaaggc ttttgagaag aagttccgct ttgattatac caatgagagg gccctgcggc
     4201 gcactctgca ggaggacctg gtgaaggacg tgctgagcaa cgcacacatc cagaacgagt
     4261 tggagcggga atttgagcgg atgcgggagg atcgggaggt gctcagggtc atcttcccaa
     4321 ctggagacag caaggtcgtc ctcccctgta acctgctgcg gatgatctgg aatgctcaga
     4381 aaatcttcca catcaaccca cgccttccct ccgacctgca ccccatcaaa gtggtggagg
     4441 gagtcaagga attgagcaag aagctggtga ttgtgaatgg ggatgaccca ctaagtcgac
     4501 aggcccagga aaatgccacg ctgctcttca acatccacct gcggtccacg ttgtgttccc
     4561 gccgcatggc agaggagttt cggctcagtg gggaggcctt cgactggctg cttggggaga
     4621 ttgagtccaa gttcaaccaa gccattgcgc atcccgggga aatggtgggg gctctggctg
     4681 cgcagtccct tggagaacct gccacccaga tgaccttgaa taccttccac tatgctggtg
     4741 tgtctgccaa gaatgtgacg ctgggtgtgc cccgacttaa ggagctcatc aacatttcca
     4801 agaagccaaa gactccttcg cttactgtct tcctgttggg ccagtccgct cgagatgctg
     4861 agagagccaa ggatattctg tgccgtctgg agcatacaac gttgaggaag gtgactgcca
     4921 acacagccat ctactatgac cccaaccccc agagcacggt ggtggcagag gatcaggaat
     4981 gggtgaatgt ctactatgaa atgcctgact ttgatgtggc ccgaatctcc ccctggctgt
     5041 tgcgggtgga gctggatcgg aagcacatga ctgaccggaa gctcaccatg gagcagattg
     5101 ctgaaaagat caatgctggt tttggtgacg acttgaactg catctttaat gatgacaatg
     5161 cagagaagct ggtgctccgt attcgcatca tgaacagcga tgagaacaag atgcaagagg
     5221 aggaagaggt ggtggacaag atggatgatg atgtcttcct gcgctgcatc gagtccaaca
     5281 tgctgacaga tatgaccctg cagggcatcg agcagatcag caaggtgtac atgcacttgc
     5341 cacagacaga caacaagaag aagatcatca tcacggagga tggggaattc aaggccctgc
     5401 aggagtggat cctggagacg gacggcgtga gcttgatgcg ggtgctgagt gagaaggacg
     5461 tggaccccgt acgcaccacg tccaatgaca ttgtggagat cttcacggtg ctgggcattg
     5521 aagccgtgcg gaaggccctg gagcgggagc tgtaccacgt catctccttt gatggctcct
     5581 atgtcaatta ccgacacttg gctctcttgt gtgataccat gacctgtcgt ggccacttga
     5641 tggccatcac ccgacacgga gtcaaccgcc aggacacagg accactcatg aagtgttcct
     5701 ttgaggaaac ggtggacgtg cttatggaag cagccgcaca cggtgagagt gaccccatga
     5761 agggggtctc tgagaatatc atgctgggcc agctggctcc ggccggcact ggctgctttg
     5821 acctcctgct tgatgcagag aagtgcaagt atggcatgga gatccccacc aatatccccg
     5881 gcctgggggc tgctggaccc accggcatgt tctttggttc agcacccagt cccatgggtg
     5941 gaatctctcc tgccatgaca ccttggaacc agggtgcaac ccctgcctat ggcgcctggt
     6001 cccccagtgt tgggagtgga atgaccccag gggcagccgg cttctctccc agtgctgcgt
     6061 cagatgccag cggcttcagc ccaggttact cccctgcctg gtctcccaca ccgggctccc
     6121 cggggtcccc aggtccctca agcccctaca tcccttcacc aggtggtgcc atgtctccca
     6181 gctactcgcc aacgtcacct gcctacgagc cccgctctcc tgggggctac acaccccaga
     6241 gtccctctta ttcccccact tcaccctcct actcccctac ctctccatcc tattctccaa
     6301 ccagtcccaa ctatagtccc acatcaccca gctattcgcc aacgtcaccc agctactcac
     6361 cgacctctcc cagctactca cccacctctc ccagctactc gcccacctct cccagctact
     6421 cgcccacctc tcccagctac tcacccactt cccctagcta ctcgcccact tcccctagct
     6481 actcgccaac gtctcccagc tactcgccga catctcccag ctactcgcca acttcaccca
     6541 gctattctcc cacttctccc agctactcac ctacctctcc aagctattca cccacctccc
     6601 ccagctactc acccacttcc ccaagttact cacccaccag cccgaactat tctccaacca
     6661 gtcccaatta caccccaaca tcacccagct acagcccgac atcacccagc tattcaccta
     6721 ctagtcccaa ctacacacct accagcccta actacagccc aacctctcca agctactctc
     6781 caacatcacc cagctattcc ccgacctcac caagttactc cccttccagc ccacgataca
     6841 caccacagtc tccaacctat accccaagct cacccagcta cagccccagc tcgcccagct
     6901 acagcccaac ctcacccaag tacaccccaa ccagtccttc ttacagtccc agctccccag
     6961 agtatacccc aacctctccc aagtactcac ctaccagtcc caaatattca cccacctctc
     7021 ccaagtactc gcctaccagt cccacctatt cacccaccac cccaaaatac tccccaacat
     7081 ctcctactta ttccccaacc tctccagtct acaccccaac ctctcccaag tactcaccta
     7141 ctagccccac ttactcgccc acttccccca agtactcgcc caccagcccc acctactcgc
     7201 ccacctcccc caaaggctca acctactctc ccacttcccc tggttactcg cccaccagcc
     7261 ccacctacag tctcacaagc ccggctatca gcccggatga cagtgacgag gagaacgcta
     7321 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     7381 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     7441 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7501 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg
     7561 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     7621 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     7681 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     7741 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     7801 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     7861 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     7921 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     7981 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     8041 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8101 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     8161 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     8221 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     8281 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     8341 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8401 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     8461 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     8521 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     8581 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     8641 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     8701 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     8761 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     8821 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     8881 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     8941 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     9001 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     9061 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     9121 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     9181 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     9241 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     9301 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     9361 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     9421 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     9481 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     9541 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     9601 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     9661 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     9721 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     9781 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9841 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9901 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9961 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    10021 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    10081 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    10141 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    10201 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    10261 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10321 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10381 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10441 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10501 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10561 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    10621 tggataaccg tattaccgcc atgcat
//
