LOCUS       sequence-142396-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OriT containing mobilizable vector for thiostrepton induced gene 
            expression in Streptomyces.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Muth G
  TITLE     The pSG5-based thermosensitive vector family for genome editing and 
            gene expression in actinomycetes.
  JOURNAL   Appl Microbiol Biotechnol. 2018 Nov;102(21):9067-9080. doi: 
            10.1007/s00253-018-9334-5. Epub 2018 Sep 6.
  PUBMED    30191290
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-018-9334-5"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2018-11"; volume: "102"; issue: "21"; pages: 
            "9067-9080"
COMMENT     Sequence Label: pGM1190
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    134..190
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      complement(338..386)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 gatcggggat ctgggctgag ggagccgacg gcacgcggcg gctcacggcg tggcacgcgg
       61 aacgtccggg cttgcacctc acgtcacgtg aggaggcagc gtggacggcg tcagagaagg
      121 gagcggacat atgaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga
      181 gctcgaattc cccagatcta aagttttgtc gtctttccag acgttagtaa atgaattttc
      241 tgtatgaggt tttgctaaac aactttcaac agtttcagcg gagtgagaat agaaaggaac
      301 aactaaagga attgcgaata ataatttttt cacgttgaaa atctccaaaa aaaaaggctc
      361 caaaaggagc ctttaattgt atcggtttat cagcttgctt tcgaggtgaa tttcttaaac
      421 agcttgatac cgatagttgc gccgacaatg acaacaacca tcgcccacgc ataaccgata
      481 tattcggtcg ctgaggcttg cagggagtca aaggccgctt ttgcgggatc tcgtcgaagg
      541 cactagaggc ctatatgcgg ggatcgaccg cgcgggtccc ggacggggaa gagcggggag
      601 ctttgccaga gagcgacgac ttccccttgc gttggtgatt gccggtcagg gcagccatcc
      661 gccatcgtcg cgtagggtgt cacaccccag gaatcgcgtc actgaacaca gcagccggta
      721 ggacgaccat gactgagttg gacaccatcg caaatccgtc cgatcccgcg gtgcagcgga
      781 tcatcgatgt caccaagccg tcgcgatcca acataaagac aacgttgatc gaggacgtcg
      841 agcc
//
