LOCUS       sequence-142399-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes for the QF-GR recombinant gene, splice linker, and mCherry 
            reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Monsalve GC, Yamamoto KR, Ward JD
  TITLE     A New Tool for Inducible Gene Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2019 Feb;211(2):419-430. doi: 10.1534/genetics.118.301705.
            Epub 2018 Nov 30.
  PUBMED    30504365
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.118.301705"; journalName: "Genetics"; date: 
            "2019-02"; volume: "211"; issue: "2"; pages: "419-430"
COMMENT     Sequence Label: pGM32DEST
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..148
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(173..178,179..196,197..203)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             257..916
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(323..342)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtggtggaat tctgcagata tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
       61 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
      121 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
      181 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
      241 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
      301 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
      361 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
      421 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
      481 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
      541 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
      601 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
      661 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
      721 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
      781 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
      841 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
      901 gcagggcggg gcgtaa
//
