LOCUS       sequence-142401-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes for the QF-GR recombinant gene, splice linker, and mCherry 
            reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Monsalve GC, Yamamoto KR, Ward JD
  TITLE     A New Tool for Inducible Gene Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2019 Feb;211(2):419-430. doi: 10.1534/genetics.118.301705.
            Epub 2018 Nov 30.
  PUBMED    30504365
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.118.301705"; journalName: "Genetics"; date: 
            "2019-02"; volume: "211"; issue: "2"; pages: "419-430"
COMMENT     Sequence Label: pGM32DEST
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    411..535
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(678..697)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agccggctgg attccatcac acttttcacg tgcagctcga cactgatcac aggccctcga
       61 gactctctgg cgtttcgaac gagcgggctc gacaagtaac gtcgtcgtgg tagggccgag
      121 gccgggtgtg tcaatgttgg tcaagtccgc aaaacgggcg ttgttgctgc ttgctgcagt
      181 gttagcgaca tgaccatcag cattgccatc agcatggcca tcagcgtggg cattggcttc
      241 ggcagcggca ttgagtgtct tgcgttttgg tggcatggct agccaagggt cctcctgaaa
      301 atgttctatg ttatgttagt atcattcgaa acataccttt gggtcctttg gccaatcccg
      361 gggatcctct agaggcgcgc ctcgagcggc cgccactgtg ctggatatca accactttgt
      421 acaagaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
      481 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggtcga
      541 cctgcagact ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta
      601 atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg
      661 gagaccggca cactggccat atcggtggtc atcatgcgcc agcttt
//
