LOCUS       sequence-142405-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Circuit backbone plasmid (LacZa insert).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Nielsen AA, Der BS, Shin J, Vaidyanathan P, Paralanov V, Strychalski
            EA, Ross D, Densmore D, Voigt CA
  TITLE     Genetic circuit design automation.
  JOURNAL   Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
  PUBMED    27034378
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-04-1"; volume: "352(6281):aac7341. doi"; pages: " 
            10.1126/science.aac7341"
COMMENT     Sequence Label: pAN1201
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     304..326
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     318..335
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     319..335
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    336..392
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(405..421)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(405..421)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(418..440)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    429..445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(453..459,460..477,478..483))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    498..519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        628..662
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     RBS             671..682
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tgatgatata tttttatctt gtgcaatgta catcagagat tttgagacac aaccaattat
       61 tgaaggcctc cctaacgggg ggcctttttt tgtttctggt ctcccgcttc tgtcttctgc
      121 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      181 attagccatt caggctgcgc aactgttggg aagggcgatc ggtgtgggcc tcttcgctat
      241 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      301 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      361 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
      421 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      481 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      541 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      601 aagacagaat ggtcaccata tatcaagttt acggctagct cagtcctagg tactatgcta
      661 gctactagag aaagaggaga aatactagat ggctgaagcg caaaatgatc ccctgctgcc
      721 gggatactcg tttaatgccc atctggtggc gggtt
//
