LOCUS       sequence-142408-       11792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Circuit plasmid: 0x41.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Nielsen AA, Der BS, Shin J, Vaidyanathan P, Paralanov V, Strychalski
            EA, Ross D, Densmore D, Voigt CA
  TITLE     Genetic circuit design automation.
  JOURNAL   Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
  PUBMED    27034378
REFERENCE   2  (bases 1 to 11792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-04-1"; volume: "352(6281):aac7341. doi"; pages: " 
            10.1126/science.aac7341"
COMMENT     Sequence Label: pAN3945
FEATURES             Location/Qualifiers
     source          1..11792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(308..852)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(388..407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(941..1756)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(941..1756)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1664..1683
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1748..1767
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        2846..3130
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     protein_bind    3171..3189
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3196..3214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5848..5866
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5873..5891
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(6744..6749,6750..6765,6766..6772)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    6778..6797
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        7605..7889
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     promoter        8770..8804
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     RBS             8813..8824
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             9680..9691
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             9809..10891
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9828..9847)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             10910..11533
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(10983..11002)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(11716..11735)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcttcctcgc tcactgactc gctgcacgag gcagacctca gcgctagcgg agtgtatact
       61 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      121 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      181 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      241 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      301 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      361 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct
      421 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
      481 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
      541 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
      601 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
      661 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
      721 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
      781 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
      841 aaacgatctc aagaagatca tcttattaag gggtctgacg ctcagtggaa cgaaaaatca
      901 atctaaagta tatatgagta aacttggtct gacagttacc ttagaaaaac tcatcgagca
      961 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     1021 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     1081 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     1141 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     1201 aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     1261 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     1321 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     1381 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     1441 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     1501 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     1561 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     1621 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     1681 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc
     1741 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     1801 ttcatgatga tatattttta tcttgtgcaa tgtacatcag agattttgag acacaaccaa
     1861 ttattgaagg cctccctaac ggggggcctt tttttgtttc tggtctcccg ctttctatga
     1921 ttggtccaga ttcgttacca attgacagct agctcagtcc taggtatata catacatgct
     1981 tgtttgtttg taaacagcgc gggtgagagg gattcgttac caattgacaa ttgattggac
     2041 gttcaatata atgctagcct gaagcggtca acgcatgtgc tttgcgttct gatgagacag
     2101 tgatgtcgaa accgcctcta caaataattt tgtttaactt tacgagggcg atcctatggc
     2161 acgtaccccg agccgtagca gcattggtag cctgcgtagt ccgcataccc ataaagcaat
     2221 tctgaccagc accattgaaa tcctgaaaga atgtggttat agcggtctga gcattgaaag
     2281 cgttgcacgt cgtgccggtg caagcaaacc gaccatttat cgttggtgga ccaataaagc
     2341 agcactgatt gccgaagtgt atgaaaatga aagcgaacag gtgcgtaaat ttccggatct
     2401 gggtagcttt aaagccgatc tggattttct gctgcgtaat ctgtggaaag tttggcgtga
     2461 aaccatttgt ggtgaagcat ttcgttgtgt tattgcagaa gcacagctgg accctgcaac
     2521 cctgacccag ctgaaagatc agtttatgga acgtcgtcgt gagatgccga aaaaactggt
     2581 tgaaaatgcc attagcaatg gtgaactgcc gaaagatacc aatcgtgaac tgctgctgga
     2641 tatgattttt ggtttttgtt ggtatcgcct gctgaccgaa cagctgaccg ttgaacagga
     2701 tattgaagaa tttaccttcc tgctgattaa tggtgtttgt ccgggtacac agcgttaagg
     2761 aaacacagaa aaaagcccgc acctgacagt gcgggctttt tttttcgacc aaaggtgtca
     2821 cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc
     2881 cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa
     2941 agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat
     3001 aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata
     3061 gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca actctctact
     3121 gtttctccat acccgttttt ttgggctagc tactccaccg ttggcttttt tccctatcag
     3181 tgatagagat tgacatccct atcagtgata gagataatga gcacctgaag cgctcaacgg
     3241 gtgtgcttcc cgttctgatg agtccgtgag gacgaaagcg cctctacaaa taattttgtt
     3301 taagagtcta tggactatgt tttcacatat gagataccag gatggcacgt aaaaccgcag
     3361 cagaagcaga agaaacccgt cagcgtatta ttgatgcagc actggaagtt tttgttgcac
     3421 agggtgttag tgatgcaacc ctggatcaga ttgcacgtaa agccggtgtt acccgtggtg
     3481 cagtttattg gcattttaat ggtaaactgg aagttctgca ggcagttctg gcaagccgtc
     3541 agcatccgct ggaactggat tttacaccgg atctgggtat tgaacgtagc tgggaagcag
     3601 ttgttgttgc aatgctggat gcagttcata gtccgcagag caaacagttt agcgaaattc
     3661 tgatttatca gggtctggat gaaagcggtc tgattcataa tcgtatggtt caggcaagcg
     3721 atcgttttct gcagtatatt catcaggttc tgcgtcatgc agttacccag ggtgaactgc
     3781 cgattaatct ggatctgcag accagcattg gtgtttttaa aggtctgatt accggtctgc
     3841 tgtatgaagg tctgcgtagc aaagatcagc aggcacagat tatcaaagtt gcactgggta
     3901 gcttttgggc actgctgcgt gaaccgcctc gttttctgct gtgtgaagaa gcacagatta
     3961 aacaggtgaa atccttcgaa taattcagcc aaaaaactta agaccgccgg tcttgtccac
     4021 taccttgcag taatgcggtg gacaggatcg gcggttttct tttctcttct caatctgcga
     4081 cgtacggtgg aatctgattc gttaccaatt gacatgatac gaaacgtacc gtatcgttaa
     4141 ggtaccagga atctgaacga ttcgttacca attgacatat ttaaaattct tgtttaaaat
     4201 gctagcctga gactgtcgcc ggatgtgtat ccgacctgac gatggcccaa aagggccgaa
     4261 acagtcctct acaaataatt ttgtttaact atggactatg tttttcaaag acgaaaaact
     4321 actagatgga aagcaccccg accaaacaga aagcaatttt tagcgcaagc ctgctgctgt
     4381 ttgcagaacg tggttttgat gcaaccacca tgccgatgat tgcagaaaat gcaaaagttg
     4441 gtgcaggcac catttatcgc tatttcaaaa acaaagaaag cctggtgaac gaactgtttc
     4501 agcagcatgt taatgaattt ctgcagtgta ttgaaagcgg tctggcaaat gaacgtgatg
     4561 gttatcgtga tggctttcat cacatttttg aaggtatggt gacctttacc aaaaatcatc
     4621 cgcgtgcact gggttttatc aaaacccata gccagggcac ctttctgacc gaagaaagcc
     4681 gtctggcata tcagaaactg gttgaatttg tgtgcacctt ttttcgtgaa ggtcagaaac
     4741 agggtgtgat tcgtaatctg ccggaaaatg cactgattgc aattctgttt ggcagcttta
     4801 tggaagtgta tgaaatgatc gagaacgatt atctgagcct gaccgatgaa ctgctgaccg
     4861 gtgttgaaga aagcctgtgg gcagcactga gccgtcagag ctaactcggt accaaattcc
     4921 agaaaagaga cgctttcgag cgtctttttt cgttttggtc cgggccttgt ccaaccaaat
     4981 gattcgttac caattgacag tttctatcga tctatagata atgctagctc gtcactagag
     5041 ggcgatagtg acaaacttga caactcatca cttcctaggt ataatgctag cctgaagaag
     5101 tcaattaatg tgcttttaat tctgatgagt cggtgacgac gaaacttcct ctacaaataa
     5161 ttttgtttaa ccccccgagg agtagcacat gccgaaactg ggtatgcaga gcattcgtcg
     5221 tcgtcagctg attgatgcaa ccctggaagc aattaatgaa gttggtatgc atgatgcaac
     5281 cattgcacag attgcacgtc gtgccggtgt tagcaccggt attattagcc attatttccg
     5341 cgataaaaac ggtctgctgg aagcaaccat gcgtgatatt accagccagc tgcgtgatgc
     5401 agttctgaat cgtctgcatg cactgccgca gggtagcgca gaacagcgtc tgcaggcaat
     5461 tgttggtggt aattttgatg aaacccaggt tagcagcgca gcaatgaaag catggctggc
     5521 attttgggca agcagcatgc atcagccgat gctgtatcgt ctgcagcagg ttagcagtcg
     5581 tcgtctgctg agcaatctgg ttagcgaatt tcgtcgtgaa ctgcctcgtg aacaggcaca
     5641 agaggcaggt tatggtctgg cagcactgat tgatggtctg tggctgcgtg cagcactgag
     5701 cggtaaaccg ctggataaaa cccgtgcaaa tagcctgacc cgtcatttta tcacccagca
     5761 tctgccgacc gattaaccaa ttattgaaca cccttcgggg tgtttttttg tttctggtct
     5821 accgtaatac tccaccgttg gcttttttcc ctatcagtga tagagattga catccctatc
     5881 agtgatagag ataatgagca cctgaagggg tcagttgatg tgctttcaac tctgatgagt
     5941 cagtgatgac gaaaccccct ctacaaataa ttttgtttaa ctatggacta tgttttcaca
     6001 tacgaggggg attagatgaa caaaaccatt gatcaggtgc gtaaaggtga tcgtaaaagc
     6061 gatctgccgg ttcgtcgtcg tccgcgtcgt agtgccgaag aaacccgtcg tgatattctg
     6121 gcaaaagccg aagaactgtt tcgtgaacgt ggttttaatg cagttgccat tgcagatatt
     6181 gcaagcgcac tgaatatgag tccggcaaat gtgtttaaac attttagcag caaaaacgca
     6241 ctggttgatg caattggttt tggtcagatt ggtgtttttg aacgtcagat ttgtccgctg
     6301 gataaaagcc atgcaccgct ggatcgtctg cgtcatctgg cacgtaatct gatggaacag
     6361 catcatcagg atcatttcaa acacatacgg gtttttattc agatcctgat gaccgccaaa
     6421 caggatatga aatgtggcga ttattacaaa agcgtgattg caaaactgct ggccgaaatt
     6481 attcgtgatg gtgttgaagc aggtctgtat attgcaaccg atattccggt tctggcagaa
     6541 accgttctgc atgcactgac cagcgttatt catccggttc tgattgcaca agaagatatt
     6601 ggtaatctgg caacccgttg tgatcagctg gttgatctga ttgatgcagg tctgcgtaat
     6661 ccgctggcaa aataaccaat tattgaacac cctaacgggt gttttttttt ttttggtcta
     6721 cccgctaacg atcgttggct gtgttgacaa ttaatcatcg gctcgtataa tgtgtggaat
     6781 tgtgagcgct cacaattctg aagtagtcac cggctgtgct tgccggtctg atgagcctgt
     6841 gaaggcgaaa ctacctctac aaataatttt gtttaaaccc ccgagatgaa atacatcctg
     6901 tttgaggtgt gcgaaatggg taaaagccgt gaacagacca tggaaaatat tctgaaagca
     6961 gccaaaaaga aattcggcga acgtggttat gaaggcacca gcattcaaga aattaccaaa
     7021 gaagccaaag ttaacgttgc aatggccagc tattacttta atggcaaaga gaacctgtac
     7081 tacgaggtgt tcaaaaaata cggtctggca aatgaactgc cgaactttct ggaaaaaaac
     7141 cagtttaatc cgattaatgc cctgcgtgaa tatctgaccg tttttaccac ccacattaaa
     7201 gaaaatccgg aaattggcac cctggcctat gaagaaatta tcaaagaaag cgcacgcctg
     7261 gaaaaaatca aaccgtattt tatcggcagc ttcgaacagc tgaaagaaat tctgcaagag
     7321 ggtgaaaaac agggtgtgtt tcactttttt agcatcaacc ataccatcca ttggattacc
     7381 agcattgttc tgtttccgaa attcaaaaaa ttcatcgata gcctgggtcc gaatgaaacc
     7441 aatgatacca atcatgaatg gatgccggaa gatctggtta gccgtattat tagcgcactg
     7501 accgataaac cgaacattta aaacgcatga gaaagccccc ggaagatcac cttccggggg
     7561 cttttttatt gcgcaggtac ttttcatact cccgccattc agagaagaaa ccaattgtcc
     7621 atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc
     7681 ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac
     7741 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt
     7801 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
     7861 tttatcgcaa ctctctactg tttctccata cccgtttttt tgggctagcc tgaagggtgt
     7921 ctcaaggtgc gtaccttgac tgatgagtcc gaaaggacga aacacccctc tacaaataat
     7981 tttgtttaaa atgttcccta ataatcagca aagaggttac tagatggcag gcgcagttgg
     8041 tcgtccgcgt cgtagtgcac cgcgtcgtgc aggtaaaaat ccgcgtgaag aaattctgga
     8101 tgcaagcgca gaactgttta cccgtcaggg ttttgcaacc accagtaccc atcagattgc
     8161 agatgcagtt ggtattcgtc aggcaagcct gtattatcat tttccgagca aaaccgaaat
     8221 ctttctgacc ctgctgaaaa gcaccgttga accgagcacc gttctggcag aagatctgag
     8281 caccctggat gcaggtccgg aaatgcgtct gtgggcaatt gttgcaagcg aagttcgtct
     8341 gctgctgagc accaaatgga atgttggtcg tctgtatcag ctgccgattg ttggtagcga
     8401 agaatttgca gaatatcata gccagcgtga agcactgacc aatgtttttc gtgatctggc
     8461 aaccgaaatt gttggtgatg atccgcgtgc agaactgccg tttcatatta ccatgagcgt
     8521 tattgaaatg cgtcgcaatg atggtaaaat tccgagtccg ctgagcgcag atagcctgcc
     8581 ggaaaccgca attatgctgg cagatgcaag cctggcagtt ctgggtgcac cgctgcctgc
     8641 agatcgtgtt gaaaaaaccc tggaactgat taaacaggca gatgcaaaat aactcggtac
     8701 caaagacgaa caataagacg ctgaaaagcg tcttttttcg ttttggtcca atggtcacca
     8761 tatatcaagt ttacggctag ctcagtccta ggtactatgc tagctactag agaaagagga
     8821 gaaatactag atggctgaag cgcaaaatga tcccctgctg ccgggatact cgtttaatgc
     8881 ccatctggtg gcgggtttaa cgccgattga ggccaacggt tatctcgatt tttttatcga
     8941 ccgaccgctg ggaatgaaag gttatattct caatctcacc attcgcggtc agggggtggt
     9001 gaaaaatcag ggacgagaat ttgtttgccg accgggtgat attttgctgt tcccgccagg
     9061 agagattcat cactacggtc gtcatccgga ggctcgcgaa tggtatcacc agtgggttta
     9121 ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat ttgccaatac
     9181 ggggttcttt cgcccggatg aagcgcacca gccgcatttc agcgacctgt ttgggcaaat
     9241 cattaacgcc gggcaagggg aagggcgcta ttcggagctg ctggcgataa atctgcttga
     9301 gcaattgtta ctgcggcgca tggaagcgat taacgagtcg ctccatccac cgatggataa
     9361 tcgggtacgc gaggcttgtc agtacatcag cgatcacctg gcagacagca attttgatat
     9421 cgccagcgtc gcacagcatg tttgcttgtc gccgtcgcgt ctgtcacatc ttttccgcca
     9481 gcagttaggg attagcgtct taagctggcg cgaggaccaa cgtatcagcc aggcgaagct
     9541 gcttttgagc accacccgga tgcctatcgc caccgtcggt cgcaatgttg gttttgacga
     9601 tcaactctat ttctcgcggg tatttaaaaa atgcaccggg gccagcccga gcgagttccg
     9661 tgccggttaa taaccaatta ttgaaggccg ctaacgcagc ctttttttgt ttctggtctc
     9721 ccaatggcgg cgcgccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc
     9781 ggaagagagt caattcaggg tggtgaatat gaaaccagta acgttatacg atgtcgcaga
     9841 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
     9901 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
     9961 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
    10021 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
    10081 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
    10141 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
    10201 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
    10261 tgaccagaca cccatcaaca gtattatttt ctcccatgag gacggtacgc gactgggcgt
    10321 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
    10381 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
    10441 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
    10501 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
    10561 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
    10621 gggatacgac gataccgaag atagctcatg ttatatcccg ccgttaacca ccatcaaaca
    10681 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
    10741 ggcggtgaag ggcaatcagc tgttgccagt ctcactggtg aaaagaaaaa ccaccctggc
    10801 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    10861 acaggtttcc cgactggaaa gcgggcagtg acaatccagg aggaaaaaaa tgtccagatt
    10921 agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg
    10981 tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca
    11041 tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca
    11101 tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa
    11161 aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg
    11221 gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg
    11281 tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg
    11341 cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga
    11401 tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc
    11461 agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga
    11521 aagtgggtcc taataattgg taacgaatca gacaattgac ggctcgaggg agtagcatag
    11581 ggtttgcaga atccctgctt cgtccatttg acaggcacat tatgcatcga tgataagctg
    11641 tcaaacatga gcagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
    11701 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
    11761 tcgggctcat gagcaaatat tttatctgag gt
//
