LOCUS       sequence-142413-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes for the 5X repeat of the QUAS response element, followed by 
            the delta pes-10 minimal promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Monsalve GC, Yamamoto KR, Ward JD
  TITLE     A New Tool for Inducible Gene Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2019 Feb;211(2):419-430. doi: 10.1534/genetics.118.301705.
            Epub 2018 Nov 30.
  PUBMED    30504365
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.118.301705"; journalName: "Genetics"; date: 
            "2019-02"; volume: "211"; issue: "2"; pages: "419-430"
COMMENT     Sequence Label: pGM34DEST
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(81..87,88..105,106..111))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(136..260)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
       61 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
      121 gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
      181 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
      241 gcttttttgt acaaacttgt tgatatctgc agaattccac cacactggat ttttctaccg
      301 gtaccgtcga cgctagccaa gggtcctcct gaaaatgttc tatgttatgt tagtatcatt
      361 cgaaacatac ctttgggtcc tttggccaat cccggggatc ctctagaggc gcgccctgaa
      421 agttaaaaat tacagtataa agataaggga aaaaggaagc acagaactcc agagaagctc
      481 agaaagagga aggtgggtaa atgaatgtac caatgaatga atgaatgatg atataccaaa
      541 acctatagga cctatcgctc cgccccctac aacgctcgta atttgcaaaa aatcgatcct
      601 gcagggatat caagcttaag gataagcgat tacccgagga taagcgatta cccgagcgga
      661 taagcgatta cccgtgagga taattgttta tccgaggata agcgattacc cggatccgca
      721 tgccctagtg ggcagatctt cgaatgcatc gcgcgcaccg tacgtctcga ggaattcctg
      781 caggatatct ggatccacga agcttcccat ggtgacgtca ccggttctag atacctaggt
      841 gagctctggt accctctagt caaggcctta agtgagtcgt attacgtagc ccgggcctag
      901 ttctagacat tctctaatga aaaaatcttt cagttgaaat tgaaaatgag ttaaagttgg
      961 agtttttatt gaaaacagat ttccgtgtga ttagtgtttt tagc
//
