LOCUS       sequence-142422-         622 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Output plasmid: PPhlF-PBetI-YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Nielsen AA, Der BS, Shin J, Vaidyanathan P, Paralanov V, Strychalski
            EA, Ross D, Densmore D, Voigt CA
  TITLE     Genetic circuit design automation.
  JOURNAL   Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
  PUBMED    27034378
REFERENCE   2  (bases 1 to 622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-04-1"; volume: "352(6281):aac7341. doi"; pages: " 
            10.1126/science.aac7341"
COMMENT     Sequence Label: pAN4037
FEATURES             Location/Qualifiers
     source          1..622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        109..158
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     primer_bind     complement(253..274)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(514..533)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccaattgaca tgatacgaaa cgtaccgtat cgttaaggta gcgcgggtga gagggattcg
       61 ttaccaattg acaattgatt ggacgttcaa tataatgcta gcctgaagct gtcaccggat
      121 gtgctttccg gtctgatgag tccgtgagga cgaaacagcc tctacaaata attttgttta
      181 atactagaga aagaggggaa atactagatg gtgagcaagg gcgaggagct gttcaccggg
      241 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      301 ggcgagggcg atggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccaca
      361 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc
      421 ttcgcccgct accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa
      481 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      541 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      601 aaggaggacg gcaacatcct gg
//
