LOCUS       sequence-142424-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Point mutation Tyr 2105 --> Ala in the FKBP12-rapamycin binding 
            (FRB) domain of mTOR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Vilella-Bach M, Nuzzi P, Fang Y, Chen J
  TITLE     The FKBP12-rapamycin-binding domain is required for 
            FKBP12-rapamycin-associated protein kinase activity and G1 
            progression.
  JOURNAL   J Biol Chem. 1999 Feb 12. 274(7):4266-72.
  PUBMED    9933627
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Feb 12. 274(7):4266-72."
COMMENT     Sequence Label: pGEX-2T FRB Y2105A
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..308
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYAHVFRRISK"
     primer_bind     complement(372..394)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(539..557)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        625..729
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgcgtggatc cgagctgatc cgagtggcca tcctctggca tgagatgtgg catgaaggcc
       61 tggaagaggc atctcgtttg tactttgggg aaaggaacgt gaaaggcatg tttgaggtgc
      121 tggagccctt gcatgctatg atggaacggg gcccccagac tctgaaggaa acatccttta
      181 atcaggccta tggtcgagat ttaatggagg cccaagagtg gtgcaggaag tacatgaaat
      241 cagggaatgt caaggacctc acccaagcct gggacctcta tgctcatgtg ttccgacgaa
      301 tctcaaagca gtagaattca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat
      361 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      421 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      481 gcagccatga cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc
      541 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
      601 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      661 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      721 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      781 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
//
