LOCUS       sequence-142428-         644 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Point mutation Trp 2027 --> Phe in the FKBP12-rapamycin binding 
            (FRB) domain of mTOR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Vilella-Bach M, Nuzzi P, Fang Y, Chen J
  TITLE     The FKBP12-rapamycin-binding domain is required for 
            FKBP12-rapamycin-associated protein kinase activity and G1 
            progression.
  JOURNAL   J Biol Chem. 1999 Feb 12. 274(7):4266-72.
  PUBMED    9933627
REFERENCE   2  (bases 1 to 644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Feb 12. 274(7):4266-72."
COMMENT     Sequence Label: pGEX-2T-FRB-W2027F
FEATURES             Location/Qualifiers
     source          1..644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..322
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMFHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     primer_bind     complement(386..408)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(553..571)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aatcggatct ggttcgcgtg gatccgagct gatccgagtg gccatcctct ggcatgagat
       61 gttccatgaa ggcctggaag aggcatctcg tttgtacttt ggggaaagga acgtgaaagg
      121 catgtttgag gtgctggagc ccttgcatgc tatgatggaa cggggccccc agactctgaa
      181 ggaaacatcc tttaatcagg cctatggtcg agatttaatg gaggcccaag agtggtgcag
      241 gaagtacatg aaatcaggga atgtcaagga cctcacccaa gcctgggacc tctattatca
      301 tgtgttccga cgaatctcaa agcagtagaa ttcatcgtga ctgactgacg atctgcctcg
      361 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
      421 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
      481 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tataattctt
      541 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      601 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cgga
//
