LOCUS       sequence-142429-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Point mutation Trp 2027 --> Phe in the FKBP12-rapamycin binding 
            (FRB) domain of mTOR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Vilella-Bach M, Nuzzi P, Fang Y, Chen J
  TITLE     The FKBP12-rapamycin-binding domain is required for 
            FKBP12-rapamycin-associated protein kinase activity and G1 
            progression.
  JOURNAL   J Biol Chem. 1999 Feb 12. 274(7):4266-72.
  PUBMED    9933627
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Feb 12. 274(7):4266-72."
COMMENT     Sequence Label: pGEX-2T-FRB-W2027F
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..320)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMFHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             complement(339..356)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(383..405)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 aaggcagaaa tcggtcagtc agtcacgatg aattctactg gctttgagat tcgtcggaac
       61 acatgataat agaggtccca ggcttgggtg aggtccttga cattccctga tttcatgtac
      121 ttcctgcacc actcttgggc ctccattaaa tctcgaccat aggcctgatt aaaggatgtt
      181 tccttcagag tctgggggcc ccgttccatc atagcatgca agggctccag cacctcaaac
      241 atgcctttca cgttcctttc cccaaagtac aaacgagatg cctcttccag gccttcatgg
      301 aacatctcat gccagaggat ggccactcgg atcagctcgg atccacgcgg aaccagatcc
      361 gattttggag gatggtcgcc accaccaaac gtggcttgcc agccctgcaa aggccatgct
      421 atatacttgc tggatttcaa gtacttatca atttgtggga tagcttcaat acgtttttta
      481 aaacaaacta attttgggaa cgcatccagg cacattgggt ccatgtataa aacaacatca
      541 agagcgtcat acaacatgaa gtcaggatgg gttacatgat caccatttaa atatgtttta
      601 tgacataaac gatcttcgaa cattttcagc atttcaggta gcttgctaag aaaatcaact
      661 ttgagagttt caaagtcttt actatatgca attctcgaaa caccgtatct aatatccaaa
      721 accgctcctt caagcattga aatctctgca cgctcttttg gacaaccacc caacatgttg
      781 tgcttgtcag ctatataacg tatgatggcc atagactgtg ttaatttaac atcaccatca
      841 atataataag gaagattggg
//
