LOCUS       sequence-142436-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes for the QS repressor gene, splice linker, and mCherry 
            reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Monsalve GC, Yamamoto KR, Ward JD
  TITLE     A New Tool for Inducible Gene Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2019 Feb;211(2):419-430. doi: 10.1534/genetics.118.301705.
            Epub 2018 Nov 30.
  PUBMED    30504365
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.118.301705"; journalName: "Genetics"; date: 
            "2019-02"; volume: "211"; issue: "2"; pages: "419-430"
COMMENT     Sequence Label: pGM48DEST
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    27..151
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(176..181,182..199,200..206)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(326..345)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agtgtggtgg aattctgcag atatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt
       61 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat
      121 actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac
      181 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc
      241 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      301 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      361 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      421 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
      481 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
      541 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
      601 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
      661 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
      721 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
      781 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
      841 cgt
//
