LOCUS       sequence-142458-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mouse eGFP-IKK-alpha in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Marazioti A, Lilis I, Vreka M, Apostolopoulou H, Kalogeropoulou A, 
            Giopanou I, Giotopoulou GA, Krontira AC, Iliopoulou M, Kanellakis 
            NI, Agalioti T, Giannou AD, Jones-Paris C, Iwakura Y, Kardamakis D, 
            Blackwell TS, Taraviras S, Spella M, Stathopoulos GT
  TITLE     Myeloid-derived interleukin-1beta drives oncogenic KRAS-NF-kappaBeta
            addiction in malignant pleural effusion.
  JOURNAL   Nat Commun. 2018 Feb 14;9(1):672. doi: 10.1038/s41467-018-03051-z.
  PUBMED    29445180
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-02-14"; volume: "9(1):672. doi"; pages: " 
            10.1038/s41467-018-03051-z"
COMMENT     Sequence Label: pEGFP.mIKK-alpha
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      153..162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      157..166
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             457..465
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtta tctagatccg
      121 gtggatcccg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
      181 ttcattctgc taaccaactc caatcaagac tcatcaaact actgctctgg tcctcatttg
      241 cttcacgaat aatagtactt aaatggccaa gacagttcag attttcttct atcatttgtg
      301 ctaacgtctc tccatcttga ggagttacca catatgtcag aggatgttca cggtctgcta
      361 atgtaggggg cagccatgct gatactggag gggttactgt gccttctaga ctggatccta
      421 caagagagcg ggcagaactc tgtgtacagg caattttaag gaggtgccaa atttctttct
      481 gcctctttcc ctgcataaac atgacagtat tgtcagcttc tttgatgtta ctgagggcca
      541 cttccacctt ggggagtaga tcaataatct tctgcttgca gcccaacaac ttgctcaggt
      601 gaccaaacag ctccttgaga acacggtcct gactctgcac ggtgtgcacg atgatcttca
      661 ccatctctgt gctgtcgctg tacaagtgat caggaggtct gtgctttagc tgcttataga
      721 gatcaatggc acgctgctcc agagactcca tcaagtctcc ctggcgtcgt ccgtaggggc
      781 tcttctgcag ctccatgatt tcagtgtgca aagacataat ttgatcctca agataaccaa
      841 tgacaccaac ctcagaatag tgaatggcct tttcttccat ttctttccat gcttttaaca
      901 ttt
//
