LOCUS       sequence-142463-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene targeting vector for the mouse Rosa26 locus, including a CAG 
            promoter, loxP flanked stop cassette, GFP, for gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Chu VT, Weber T, Graf R, Sommermann T, Petsch K, Sack U, Volchkov P,
            Rajewsky K, Kuhn R
  TITLE     Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in 
            C57BL/6 zygotes.
  JOURNAL   BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4.
  PUBMED    26772810
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-01-16"; volume: "16(1):4. doi"; pages: " 
            10.1186/s12896-016-0234-4"
COMMENT     Sequence Label: pR26 CAG/GFP Dest
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    30..77
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(102..118)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(131..148)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    137..361
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agtaatccgg gatccggaga gctcccaacg aagttcctat tccgaagttc ctattctcta
       61 gaaagtatag gaacttcctc gaggttggat gcagcccggg ggatccacta gttctagagc
      121 ggccgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
      181 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
      241 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
      301 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
      361 gcttctgagg cggaaagaac cagctggggc tcgagatcca ctagttctag cctcgaggct
      421 agagcggccg ccgcgccgat atcgaattcg ctagaagatg ggcgggagtc ttctgggcag
      481 gcttaaaggc taacctggtg tgtgggcgtt gtcctgcagg ggaattgaac aggtgtaaaa
      541 ttggagggac aagacttccc acagattttc ggttttgtcg ggaagttttt taataggggc
      601 aaataaggaa aatgggagga taggtagtca tctggggttt tatgcagcaa aactacaggt
      661 tattattgct tgtgatccgc ctcggagtat tttccatcga ggtagattaa agacatgctc
      721 acccgagttt tatactctcc tgcttgagat ccttactaca gtatgaaatt acagtgtc
//
