LOCUS       sequence-142470-        6251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express WDR41 HA tagged in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6251)
  TITLE     pcDNA3 HA C9ORF72 op
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV6 WDR41 HA
FEATURES             Location/Qualifiers
     source          1..6251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2423..2449
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2465..2488
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2508..2524)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2508..2524)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2535..3157
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2734..2753)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      complement(3306..3894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3386..3405)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4223..4270
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4295..4314
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4502..5296)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4502..5296)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4613..4632)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5223..5242
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5331..5688)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5345..5480
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5497..5688
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5645..5665
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(5690..5794)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5821..6249
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5908..5927)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6118..6139
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgccatgttg cgatggctga tcgggggagg ccgagaaccg cagggactgg ccgagaaatc
     1081 tcctttacag acaataggtg aagaacaaac ccagaatccc tacactgaac tgctagtact
     1141 gaaggctcat catgatattg tacgatttct ggtacagtta gatgactaca gatttgcatc
     1201 tgctggtgat gatggaattg tagttgtgtg gaatgcccag acaggggaaa aacttttaga
     1261 actgaatgga cacactcaaa agataacagc tattattaca tttccttcct tggaatcttg
     1321 tgaagagaaa aatcaactca tcttgacagc ctctgctgat agaacagtta ttgtgtggga
     1381 tggtgatact accagacaag ttcagagaat atcatgcttc cagtctactg taaagtgttt
     1441 aactgttctt cagagactag atgtttggct ttctggtggg aatgacctgt gtgtgtggaa
     1501 ccgaaaatta gatctcctgt gtaagactag ccacctttct gatacaggta ttagtgcttt
     1561 ggttgaaata cctaagaact gtgttgtggc agcagttggc aaagaactga taattttcag
     1621 gttggtagca cccacagaag gatcactaga atgggatatt cttgaagtta agcgcctcct
     1681 tgatcaccag gataatattc tctcattgat taatgtcaat gatttgagtt ttgtcaccgg
     1741 ctcccacgtc ggagagctga tcatctggga tgccctggac tggaccatgc aggcctatga
     1801 acgcaacttc tgggacccat ctccacaact ggacacccaa caagaaataa aactctgtca
     1861 aaaatcaaat gacatttcta ttcatcattt cacatgtgat gaagagaatg tatttgctgc
     1921 agttggaagg ggtttatacg tgtatagcct tcaaatgaag cgtgtgattg cctgccagaa
     1981 aactgcacat gactccaatg tcctgcacgt tgccagactt ccaaacaggc agttaatctc
     2041 atgctcagaa gatggcagtg tacgcatttg ggagttaaga gaaaaacagc agcttgcagc
     2101 tgagcctgta ccaacaggtt tttttaacat gtggggattt ggaagagtca gcaaacaagc
     2161 cagccaacct gttaaaaagc agcaagaaaa tgctacttca tgttcactgg agcttattgg
     2221 agatttgatt ggacactcat catctgtgga gatgtttcta tactttgaag atcatggact
     2281 agtgacgtgc tccgctgatc atctcattat tttgtggaaa aatggagagc gagaatctgg
     2341 attgcgcagt ttaagattat ttcaaaaatt agaggagaat ggtgacttat accttgctgt
     2401 cacgcgtacg cggccgctcg agtacccata cgatgttcca gattacgcta agtaagatat
     2461 cctggattac aaggatgacg acgataaggt ttaaacggcc ggccgcggtc atagctgttt
     2521 cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
     2581 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
     2641 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
     2701 aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
     2761 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
     2821 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
     2881 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
     2941 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
     3001 tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag acacagcata
     3061 ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc actgtcctct
     3121 catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca gcggcgagcg
     3181 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3241 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3301 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3361 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3421 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3481 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3541 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3601 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3661 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3721 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3781 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3841 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3901 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3961 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4021 tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc tgccgccccg
     4081 acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa
     4141 gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga gtgccagccc
     4201 tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt attctgtctt
     4261 tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc
     4321 ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag
     4381 ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg
     4441 aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg
     4501 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
     4561 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
     4621 gggtagccaa cgctatgtcc tgatagcgat ccgccacacc cagccggcca cagtcgatga
     4681 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
     4741 cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg
     4801 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
     4861 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
     4921 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
     4981 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
     5041 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
     5101 gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
     5161 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
     5221 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
     5281 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag
     5341 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
     5401 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     5461 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     5521 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     5581 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     5641 ccacacccta actgacacac attccacagc tggttctttc cgcctcagga ctcttccttt
     5701 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5761 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5821 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5881 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5941 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6001 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6061 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6121 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6181 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6241 acgcgaattt t
//
