LOCUS       sequence-142475-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX_403
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(103..227)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             232..273
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             287..304
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     743..761
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
       61 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
      121 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
      181 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttgc aggaaagcca
      241 atcccaaacc cactcctagg tctcgacagt acagcataga ccggtccacc accaccacca
      301 ccactaagga tccggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg
      361 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
      421 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
      481 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
      541 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
      601 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
      661 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
      721 gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg
      781 cagtcggctc c
//
