LOCUS       sequence-142477-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation, Inducible lentiviral expression, TRE-gateway; 
            PGK-rtTA-2A-puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW57.1
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(80..86,87..104,105..110))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(135..259)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(280..594)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(283..307)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    354..372
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    390..408
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    425..443
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    461..479
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    497..515
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    532..550
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    568..586
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacgga
       61 tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg
      121 cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
      181 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
      241 cttttttgta caaacttgtt gatgctagcc aattctccag gcgatctgac ggttcactaa
      301 acgagctctg cttatatagg cctcccaccg tacacgccta cctcgacata cgttctctat
      361 cactgatagg gagtaaactc gacatacgtt ctctatcact gatagggata aactcgacat
      421 acgttctcta tcactgatag ggagtaaact cgacatacgt tctctatcac tgatagggag
      481 taaactcgac atacgttctc tatcactgat agggagtaaa ctcgacatcg ttctctatca
      541 ctgataggga gtaaactcga catacgttct ctatcactga tagggagtaa actcgacata
      601 tcgattcgcg gccaaagtgg atctctgctg tccctgtaat aaacccgaaa attttgaatt
      661 tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt
      721 ctttcccctg cactgtaccc
//
