LOCUS       sequence-142479-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation, Inducible lentiviral expression, TRE-gateway; 
            PGK-rtTA-2A-puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW57.1
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(87..211)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             229..246
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        256..766
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     685..703
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(776..795)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
       61 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
      121 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
      181 tttctcgttc agctttcttg tacaaagtgg tttagtaatg aaccggtcca ccaccaccac
      241 caccactaag gatccggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca
      301 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg
      361 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc
      421 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg
      481 ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca
      541 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg
      601 gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt
      661 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc
      721 ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccagcaat tcaccatgac
      781 cgagtacaag cccacggtgc gcc
//
