LOCUS       sequence-142480-         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RNF169 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Panier S, Ichijima Y, Fradet-Turcotte A, Leung CC, Kaustov L, 
            Arrowsmith CH, Durocher D
  TITLE     Tandem protein interaction modules organize the ubiquitin-dependent 
            response to DNA double-strand breaks.
  JOURNAL   Mol Cell. 2012 Aug 10;47(3):383-95. doi: 
            10.1016/j.molcel.2012.05.045. Epub 2012 Jun 27.
  PUBMED    22742833
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.05.045"; journalName: "Mol Cell"; date: 
            "2012-08-10- 10"; volume: "47"; issue: "3"; pages: "383-95"
COMMENT     Sequence Label: pcDNA5-FRT/TO-Flag-RNF169
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..32
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     42..66
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             166..189
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt agtgaaccgt
       61 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
      121 tccagcctcc ggactctagc gtttaaactt aagcttggta ccatggacta caaagacgat
      181 gacgataaag ccgccagttt aaacggcgcg ccattaatta aaatggcggc tgcaggtccg
      241 agtactcggg cctcttccgc ggcggcagca gccgctctga gtcggcgggg ccggcggggc
      301 cgctgtgacg agacggcggc agctaagact ggggccccag gcccggcttc tggaccttcg
      361 ctgttggtgt tgtcgccgcc gttgctgcag ccgccgctgc cgccgcggcc ggaggaatcg
      421 ggctgcgccg ggtgcctgga gccccccgga gaagcagcgg ccctgccgtg cggccactcg
      481 ctttgccgag gctgcgccca acgcgccgcc gacgcggcgg gcccgggttg ccctcgctgc
      541 cgcgcccgcg gcccaggctg ggcccgccgt cgggcccgcg acgacggcca ggccgactca
      601 gaggtgctgg gcgagtgcgc ccgccgcagc caacccgagc gctgccgccc gcgccgggac
      661 gggggc
//
