LOCUS       sequence-142490-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PG1424PGA
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gaatccccct gcaggtcctg tgcgtgctat
      121 cgctgagtac gaacgctctg cagccgtttt ggtacgctac ccgttcggta tcccgatgga
      181 attgatcaaa gagctggcca agaacgacaa ggtgattacc attgtggcga gtgaaagcca
      241 aaaaaacacc gttataaccc agtacaccca aagcggtgtg aatctctcta attgcgattt
      301 catcattgcg aaaactgact cttactggac acgcgactat accggttggt tcgcaatgta
      361 cgatacgaac aaagtaggtc tcgtggactt tatttataac cgccctcgtc ctaacgatga
      421 tgaattcccc aaatacgaag cacaatatct gggcatcgag atgttcggga tgaagctcaa
      481 gcagaccggt ggcaactaca tgacggacgg atatggatcc gctgtgcagt cacatatcgc
      541 atatacggag aactcctctc tgtctcaagc tcaagtaaat caaaagatga aagactatct
      601 cggcatcaca catcatgatg tggtacaaga tccgaacggc gaatatatca accatgtgga
      661 ctgttggggc aagtatttgg caccgaacaa aatcctcatc aggaaagtgc ctgacaatca
      721 ccctcagcac caagccctgg aagatatggc agcctacttc gcagcacaga cctgcgcatg
      781 ggga
//
