LOCUS       sequence-142498-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KCTD13A
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(461..481)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(506..523)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             534..556
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    571..595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(595..614)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(596..614)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(698..717)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atccgtatcc acctttactg tcacagcgtc tccctttttt
      121 gctgcagcgc cagctggcag tcctcaatca ggccctgcac caggtagtag cgtgcttcgc
      181 ccagcagctc ccccagttct ctcgtactct ccggcagtgg cacagaccca tcccgcaggt
      241 aattgaggat tgtaccaaag tgacggccgc tccggtcaat cagcacccaa cctccggcat
      301 cggtcagcac ctccacgcgg ccgctgaaca tggctttgag catggtgtcc tgtcccgtga
      361 gggtgcgcag cgtggtgtag tgcaacgagc cgcccacgtt cagcttcacg tatttgctgt
      421 tcggggtcag cggcttgaga ccgtaggcgg cggggcccat ggattggaag tacaggttct
      481 cggtacccag atctacacca gaagaatgat gatgatgatg gtgcatatgt atatctcctt
      541 cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat
      601 agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg
      661 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
      721 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
      781 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
      841 gcggcggtgc tcaacggc
//
