LOCUS       sequence-14250-e        2568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pLR-HD
FEATURES             Location/Qualifiers
     source          1..2568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             498..1289
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1382..1970
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1871..1890
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2124..2141
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2300..2327)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2419..2505)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(2554..2568,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(2568,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc ccgtctcgct atcgccagcc acgatggcgg
       61 caagcaagcg ctcgaaagca ttgtggccca gctgagccgg cctgatccgg cgttggccgc
      121 gttgaccaac gagagacgtc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
      181 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
      241 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
      301 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
      361 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
      421 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
      481 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
      541 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
      601 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
      661 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
      721 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
      781 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
      841 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
      901 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
      961 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     1021 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     1081 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     1141 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     1201 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     1261 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     1321 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1381 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1441 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1501 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     1561 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1621 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1681 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1741 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     1801 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1861 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1921 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1981 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2041 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2101 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     2161 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     2221 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     2281 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     2341 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     2401 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     2461 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     2521 ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgtt
//
