LOCUS       sequence-142500-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BRD1A
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             556..573
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(626..644)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      640..687
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat ggagcaggtc gccatggagc tgcggctgac
      121 cgaactgacg cgcctgctgc gctcagtgct ggaccagctg caagacaagg accccgccag
      181 gatatttgcg cagcccgtga gtctgaagga ggtaccagat tatttggatc acattaaaca
      241 tcccatggac tttgccacaa tgaggaaacg gttagaagct caagggtata aaaacctcca
      301 tgagtttgag gaggattttg atctcattat agataactgc atgaagtaca atgccaggga
      361 caccgtgttc tatagagccg cggtgaggct gcgcgatcag ggaggtgttg ttctgaggca
      421 ggcccggcgc gaggtggaca gcatcggctt ggaagaggcc tcggggatgc acctgcctga
      481 gcggcctgct tgacagtaaa ggtggatacg gatccgaatt cgagctccgt cgacaagctt
      541 gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga
      601 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      661 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
      721 gggacgcgcc ctgtagcggc gcattaagcg cggcgg
//
