LOCUS       sequence-142505-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SPIN3A
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(88..111)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(112..129)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(130..150)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atcctcactt atcgtcgtca tccttgtaat catggtggtg
      121 atgatggtgc gattggaagt agaggttctc tgcagatgtt tttaccaaat cgtagacata
      181 gatatggaaa tcatcatcaa atttgatgaa gtacacagag ggttttgctt ctacctgatg
      241 aatgaccatg ccagttctct tggagccatc gtctttggcg tattccacct gtttgcctac
      301 caggctgtct atgacttctc ctggctccct ctctgccaga ggagaatcat tggaatcttg
      361 aaggatgcgg aggtcaccat ctttataatc atctaagagc tggtacatat ataatacagg
      421 atctttctca taggtaatgt aaaaccatgt gttcatgaca ggtgcctgag ctaagaccat
      481 ccccctccat tcatttttgg aaccttcctc tgtctcaaaa atatgttcca ctgccttgcc
      541 aaccattatt tctgccaagt gtgtatcact gattctagat gatgcaactc tattaggaag
      601 gacttcaagt gatgacactc tttcatctct gtgaagttct aatccataaa cacagtcaaa
      661 tccatcatat ttgataagat acagagaggg atttacaggt acctgatcca gaacggttcc
      721 tttccactgt gttagaggtt catctccatc tttccatccg tgctgaattc tgcagcccac
      781 gatgttcccc cgaggcatag tatatctcct tcttaaggtt aaacaaaatt atttctagag
      841 gg
//
