LOCUS       sequence-142506-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SPIN3A
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             670..690
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             691..708
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             709..732
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accttaagaa ggagatatac tatgcctcgg gggaacatcg tgggctgcag aattcagcac
       61 ggatggaaag atggagatga acctctaaca cagtggaaag gaaccgttct ggatcaggta
      121 cctgtaaatc cctctctgta tcttatcaaa tatgatggat ttgactgtgt ttatggatta
      181 gaacttcaca gagatgaaag agtgtcatca cttgaagtcc ttcctaatag agttgcatca
      241 tctagaatca gtgatacaca cttggcagaa ataatggttg gcaaggcagt ggaacatatt
      301 tttgagacag aggaaggttc caaaaatgaa tggaggggga tggtcttagc tcaggcacct
      361 gtcatgaaca catggtttta cattacctat gagaaagatc ctgtattata tatgtaccag
      421 ctcttagatg attataaaga tggtgacctc cgcatccttc aagattccaa tgattctcct
      481 ctggcagaga gggagccagg agaagtcata gacagcctgg taggcaaaca ggtggaatac
      541 gccaaagacg atggctccaa gagaactggc atggtcattc atcaggtaga agcaaaaccc
      601 tctgtgtact tcatcaaatt tgatgatgat ttccatatct atgtctacga tttggtaaaa
      661 acatctgcag agaacctcta cttccaatcg caccatcatc accaccatga ttacaaggat
      721 gacgacgata agtgaggatc cga
//
