LOCUS       sequence-142509-         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of human POLQ -DY2230AA mutant (a polymerase 
            domain mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Yousefzadeh MJ, Wyatt DW, Takata K, Mu Y, Hensley SC, Tomida J, 
            Bylund GO, Doublie S, Johansson E, Ramsden DA, McBride KM, Wood RD
  TITLE     Mechanism of suppression of chromosomal instability by DNA 
            polymerase POLQ.
  JOURNAL   PLoS Genet. 2014 Oct 2;10(10):e1004654. doi: 
            10.1371/journal.pgen.1004654. eCollection 2014 Oct.
  PUBMED    25275444
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2014-10-2"; volume: "10(10):e1004654. doi"; pages: "
            10.1371/journal.pgen.1004654. eCollection 2014 Oct"
COMMENT     Sequence Label: pCDH-EF1-FHC-POLQ-DY2230AA
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    155..271
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(538..582)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc cgttcaattg
       61 ccgacccctc cccccaactt ctcggggact gtgggcgatg tgcgctctgc ccactgacgg
      121 gcaccggagc gatcgcagat ccactagtat cgataaaatt ttgaattttg taatttgttt
      181 ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact
      241 gtacccccca atcccccctt ttcttttaaa agttaaccga taccgtcgag atccgttcac
      301 taatcgaatg gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct
      361 gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct
      421 aactctattc actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac
      481 tatcattatg aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa
      541 acttgcccat ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga
      601 ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat
      661 ccaggtcgtg tgattccaaa tctgttccag agatttatta ctccaactag
//
