LOCUS       sequence-142510-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KCTD16A
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             511..528
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(581..599)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      595..642
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat ggggtccgca gttcccaact ccttccctga
      121 ggtggtagag ctgaatgtcg ggggtcaagt ttattttact cgccattcca cattgataag
      181 catccctcat tccctcctgt ggaaaatgtt ttccccaaag agagacacgg ctaatgatct
      241 agccaaggac tccaagggaa ggtttttcat tgacagagat ggattcttgt tccgttatat
      301 tctggactat ctcagggaca ggcaggtggt cctgcctgat cactttccag aaaaaggaag
      361 actgaaaagg gaagctgaat acttccagct cccagacttg gtcaaactcc tgacccccga
      421 tgaaatcaag caaagcccag atgaatgaca gtaaaggtgg atacggatcc gaattcgagc
      481 tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
      541 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      601 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      661 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      721 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcc
//
