LOCUS       sequence-142511-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KCTD16A
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(455..475)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(500..517)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             528..550
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    565..589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(589..608)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(590..608)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(692..711)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg
       61 gagctcgaat tcggatccgt atccaccttt actgtcattc atctgggctt tgcttgattt
      121 catcgggggt caggagtttg accaagtctg ggagctggaa gtattcagct tcccttttca
      181 gtcttccttt ttctggaaag tgatcaggca ggaccacctg cctgtccctg agatagtcca
      241 gaatataacg gaacaagaat ccatctctgt caatgaaaaa ccttcccttg gagtccttgg
      301 ctagatcatt agccgtgtct ctctttgggg aaaacatttt ccacaggagg gaatgaggga
      361 tgcttatcaa tgtggaatgg cgagtaaaat aaacttgacc cccgacattc agctctacca
      421 cctcagggaa ggagttggga actgcggacc ccatggattg gaagtacagg ttctcggtac
      481 ccagatctac accagaagaa tgatgatgat gatggtgcat atgtatatct ccttcttaaa
      541 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag
      601 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc
      661 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
      721 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
      781 cccgtggccg gg
//
