LOCUS       sequence-142526-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA tagged aa120-433 AKT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Zeng M, van der Donk WA, Chen J
  TITLE     Lanthionine synthetase C-like protein 2 (LanCL2) is a novel 
            regulator of Akt.
  JOURNAL   Mol Biol Cell. 2014 Dec 1;25(24):3954-61. doi: 
            10.1091/mbc.E14-01-0004. Epub 2014 Oct 1.
  PUBMED    25273559
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-01-0004"; journalName: "Mol Biol Cell"; date: 
            "2014-12-1- 1"; volume: "25"; issue: "24"; pages: "3954-61"
COMMENT     Sequence Label: pCDNA3-HA-Akt1-aa120-433
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             132..158
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg
      121 gaattgaaat gtacccatac gacgtcccag actacgctgg gaattcattc cggtcgggct
      181 cacccagtga caactcaggg gctgaagaga tggaggtgtc cctggccaag cccaagcacc
      241 gcgtgaccat gaacgagttt gagtacctga agctgctggg caagggcact ttcggcaagg
      301 tgatcctggt gaaggagaag gccacaggcc gctactacgc catgaagatc ctcaagaagg
      361 aagtcatcgt ggccaaggac gaggtggccc acacactcac cgagaaccgc gtcctgcaga
      421 actccaggca ccccttcctc acagccctga agtactcttt ccagacccac gaccgcctct
      481 gctttgtcat ggagtacgcc aacgggggcg agctgttctt ccacctgtcc cgggagcgtg
      541 tgttctccga ggaccgggcc cgcttctatg gcgctgagat tgtgtcagcc ctggactacc
      601 tgcactcgga gaagaacgtg gtgtaccggg acctcaagct ggagaacctc atgctggaca
      661 aggacgggca cattaagatc acagacttcg ggctgtgcaa ggaggggatc aaggacggtg
      721 ccaccatgaa gaccttttgc ggcacacctg agtacctggc ccccgaggtg ctggaggaca
      781 atgactacgg ccgtgcagtg gactggtggg ggctgggcgt ggtcatgtac gagatgatgt
      841 gcggtcgcct gcccttctac aaccaggacc atgagaagct ttttgagctc atcctcatg
//
