LOCUS       sequence-142542-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA tagged aa1-149 of AKT1
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Zeng M, van der Donk WA, Chen J
  TITLE     Lanthionine synthetase C-like protein 2 (LanCL2) is a novel 
            regulator of Akt.
  JOURNAL   Mol Biol Cell. 2014 Dec 1;25(24):3954-61. doi: 
            10.1091/mbc.E14-01-0004. Epub 2014 Oct 1.
  PUBMED    25273559
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-01-0004"; journalName: "Mol Biol Cell"; date: 
            "2014-12-1- 1"; volume: "25"; issue: "24"; pages: "3954-61"
COMMENT     Sequence Label: pCDNA3-HA-Akt1-aa1-149
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             132..158
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(642..660)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(643..660)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(680..697)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    686..797
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg
      121 gaattgaaat gtacccatac gacgtcccag actacgctgg gaattcaagc gacgtggcta
      181 ttgtgaagga gggttggctg cacaaacgag gggagtacat caagacctgg cggccacgct
      241 acttcctcct caagaatgat ggcaccttca ttggctacaa ggagcggccg caggatgtgg
      301 accaacgtga ggctcccctc aacaacttct ctgtggcgca gtgccagctg atgaagacgg
      361 agcggccccg gcccaacacc ttcatcatcc gctgcctgca gtggaccact gtcatcgaac
      421 gcaccttcca tgtggagact cctgaggagc gggaggagtg gacaaccgcc atccagactg
      481 tggctgacgg cctcaagaag caggaggagg aggagatgga cttccggtcg ggctcaccca
      541 gtgacaactc aggggctgaa gagatggagg tgtccctggc caagcccaag caccgcgtga
      601 ccatgaacga gtagctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat
      661 gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
      721 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
      781 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg g
//
