LOCUS       sequence-142544-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA tagged aa1-149 of AKT1
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Zeng M, van der Donk WA, Chen J
  TITLE     Lanthionine synthetase C-like protein 2 (LanCL2) is a novel 
            regulator of Akt.
  JOURNAL   Mol Biol Cell. 2014 Dec 1;25(24):3954-61. doi: 
            10.1091/mbc.E14-01-0004. Epub 2014 Oct 1.
  PUBMED    25273559
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-01-0004"; journalName: "Mol Biol Cell"; date: 
            "2014-12-1- 1"; volume: "25"; issue: "24"; pages: "3954-61"
COMMENT     Sequence Label: pCDNA3-HA-Akt1-aa1-149
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(490..516)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(583..602)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(584..602)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(647..851)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(676..696)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagctactcg ttcatggtca cgcggtgctt
       61 gggcttggcc agggacacct ccatctcttc agcccctgag ttgtcactgg gtgagcccga
      121 ccggaagtcc atctcctcct cctcctgctt cttgaggccg tcagccacag tctggatggc
      181 ggttgtccac tcctcccgct cctcaggagt ctccacatgg aaggtgcgtt cgatgacagt
      241 ggtccactgc aggcagcgga tgatgaaggt gttgggccgg ggccgctccg tcttcatcag
      301 ctggcactgc gccacagaga agttgttgag gggagcctca cgttggtcca catcctgcgg
      361 ccgctccttg tagccaatga aggtgccatc attcttgagg aggaagtagc gtggccgcca
      421 ggtcttgatg tactcccctc gtttgtgcag ccaaccctcc ttcacaatag ccacgtcgct
      481 tgaattccca gcgtagtctg ggacgtcgta tgggtacatt tcaattccag cacactggcg
      541 gccgttacta gtggatccga gctcggtacc aagcttgggt ctccctatag tgagtcgtat
      601 taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat
      661 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
      721 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
      781 ggagacttgg aaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
      841 aaccgcatca ccatg
//
