LOCUS       sequence-142560-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: STK10
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gcgcgaatat gagcacgtcc gccgcgacct
      121 ggaccccaac gaggtgtggg agatcgtggg cgagctgggc gacggcgcct tcggcaaggt
      181 ttacaaggcc aagaataagg agacgggtgc tttggctgcg gccaaagtca ttgaaaccaa
      241 gagtgaggag gagctggagg actacatcgt ggagattgag atcctggcca cctgcgacca
      301 cccctacatt gtgaagctcc tgggagccta ctatcacgac gggaagctgt ggatcatgat
      361 tgagttctgt ccagggggag ccgtggacgc catcatgctg gagctggaca gaggcctcac
      421 ggagccccag atacaggtgg tttgccgcca gatgctagaa gccctcaact tcctgcacag
      481 caagaggatc atccaccgag atctgaaagc tggcaacgtg ctgatgaccc tcgagggaga
      541 catcaggctg gctgactttg gtgtgtctgc caagaatctg aagactctac agaaacgaga
      601 ttccttcatc ggcacgcctt actggatggc ccccgaggtg gtcatgtgtg agaccatgaa
      661 agacacgccc tacgactaca aagccgacat ctggtccctg ggcatcacgc tgattgagat
      721 ggcccagatc gagccgccac accacgagct caaccccatg cgggtcctgc taaagatcgc
      781 caagtcagac cctcccacgc tgctca
//
