LOCUS       sequence-142567-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NUDT1
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..45
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             617..634
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(687..705)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      701..748
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac catgaaacat caccatcacc atcaccccat gagcgattac
       61 gacatcccca ctactgagaa tctttatttt cagggcgcca tgggcgcgag tcgtctgtat
      121 accctggtcc tggtcctgca accgcaacgt gtcctgctgg gcatgaaaaa acgtggcttt
      181 ggcgctggtc gttggaacgg ctttggcggc aaggtccagg aaggcgaaac cattgaagat
      241 ggtgcgcgtc gcgaactgca agaagaaagt ggtctgacgg tggacgccct gcataaagtc
      301 ggccagatcg tgtttgaatt cgttggtgaa ccggaactga tggatgtgca cgtgttttgc
      361 accgacagca ttcaaggcac gccggttgaa tctgatgaaa tgcgtccgtg ttggtttcag
      421 ctggatcaaa tcccgttcaa ggacatgtgg ccggatgaca gctattggtt cccgctgctg
      481 ctgcagaaaa agaaatttca tggttacttc aagtttcaag gtcaagatac gattctggac
      541 tacacgctgc gcgaagtgga caccgtctga ggatccgaat tcgagctccg tcgacaagct
      601 tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg
      661 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
      721 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcga
//
