LOCUS       sequence-142580-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KCTD17
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             493..510
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(563..581)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      577..624
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(748..767)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gggggcgggc ggccgcgccg caggcggctg
      121 gggcaagtgg gtgcggctca acgtgggggg cacggtgttc ctgaccaccc ggcagacgct
      181 gtgccgcgag cagaagtcct tcctcagccg cctgtgccag ggggaagagc tgcagtcgga
      241 ccgggatgag accggggcct acctcattga ccgtgacccc acctacttcg ggcccatcct
      301 gaacttcctc cggcatggca agctggtgct ggacaaggac atggctgagg agggggtcct
      361 ggaggaagcc gagttctaca acatcggccc gctgatccgc atcatcaaag accggatgga
      421 agagaagtga cagtaaaggt ggatacggat ccgaattcga gctccgtcga caagcttgcg
      481 gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      541 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      601 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
      661 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      721 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      781 cgt
//
