LOCUS       sequence-142582-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Mmadhc
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             661..678
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(731..749)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      745..792
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gcctgttgaa caagaaatta acagtgcaga
      121 aacttacttt gaaagtgcca aagttgagtg tgcgatccag acatgtccag agctgctgcg
      181 gagagatttt gagtcacttt ttccagaagt ggctaatagc aaactaatga tcctgactgt
      241 aacccagaaa actgaaaatg acatgactgt ctggagtgaa gaggtggagg ttgagagaga
      301 ggtgctcttg gaaaagttca tcagtggtgc taaggagatc tgctatgccc ttcgggctga
      361 aggatattgg gctgacttca ttgacccatc atctggtgtg gcattttttg gaccatatac
      421 caacaacaca ctttttgaaa cagatgaacg gtaccggcat ttaggattct cagttgacga
      481 ccttggctgc tgtaaagtga tccgtcacag tctctggggt actcatgtgg ttgtaggaag
      541 tatcttcact aacgcaacag cagacagcag tatcatgagg aagctcagtg gaaactagca
      601 gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag
      661 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
      721 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
      781 aggggttttt tgctgaaagg aggaactata tccggattgg cga
//
