LOCUS       sequence-142583-         573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 573)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Mmadhc
FEATURES             Location/Qualifiers
     source          1..573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(482..502)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(527..544)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgctgtctgc tgttgcgtta gtgaagatac ttcctacaac cacatgagta ccccagagac
       61 tgtgacggat cactttacag cagccaaggt cgtcaactga gaatcctaaa tgccggtacc
      121 gttcatctgt ttcaaaaagt gtgttgttgg tatatggtcc aaaaaatgcc acaccagatg
      181 atgggtcaat gaagtcagcc caatatcctt cagcccgaag ggcatagcag atctccttag
      241 caccactgat gaacttttcc aagagcacct ctctctcaac ctccacctct tcactccaga
      301 cagtcatgtc attttcagtt ttctgggtta cagtcaggat cattagtttg ctattagcca
      361 cttctggaaa aagtgactca aaatctctcc gcagcagctc tggacatgtc tggatcgcac
      421 actcaacttt ggcactttca aagtaagttt ctgcactgtt aatttcttgt tcaacaggca
      481 tggattggaa gtacaggttc tcggtaccca gatctacacc agaagaatga tgatgatgat
      541 ggtgcatatg tatatctcct tccttaaagt taa
//
