LOCUS       sequence-142587-        8845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive ETV4 ORF, blasticidin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Wang B, Krall EB, Aguirre AJ, Kim M, Widlund HR, Doshi MB, Sicinska 
            E, Sulahian R, Goodale A, Cowley GS, Piccioni F, Doench JG, Root DE,
            Hahn WC
  TITLE     ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to 
            MAPK Pathway Inhibition.
  JOURNAL   Cell Rep. 2017 Feb 7;18(6):1543-1557. doi: 
            10.1016/j.celrep.2017.01.031.
  PUBMED    28178529
REFERENCE   2  (bases 1 to 8845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.01"; journalName: "Cell Rep"; date: 
            "2017-02-7- 7"; volume: "18"; issue: "6"; pages: "1543-1557"
COMMENT     Sequence Label: pLX_TRC311 ETV4-S
FEATURES             Location/Qualifiers
     source          1..8845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1942..1966)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1974..2015
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2066..2654
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2119..2139)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2537..2548)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2726..2959
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3037..3158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3074..3093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3128..3147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3198..3333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3353..3372)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3354..3372)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3382..3399)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3382..3398)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3391..3413)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3540..3995
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3627..3646)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3837..3858
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4021..4125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4126..4194,4195..4986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4344..4363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5157..5745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5646..5665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5899..5916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6033..6054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6069..6074,6075..6092,6093..6099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6107..6123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6112..6134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6131..6147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6131..6147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6166..6186
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6168..6186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6214..6440
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6441..6621
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6668..6793
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7286..7519
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7704..7748
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7979..8189
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8001..8197
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8048..8183
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8219..8614
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8688..8805
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
     1321 tgtacctcca cacagagggc ttctctgggc cctctccagg tgacggggcc atgggctatg
     1381 gctatgagaa acctctgcga ccattcccag atgatgtctg cgttgtccct gagaaatttg
     1441 aaggagacat caagcaggaa ggggtcggtg catttcgaga ggggccgccc taccagcgcc
     1501 ggggtgccct gcagctgtgg caatttctgg tggccttgct ggatgaccca acaaatgccc
     1561 atttcattgc ctggacgggc cggggaatgg agttcaagct cattgagcct gaggaggtcg
     1621 ccaggctctg gggcatccag aagaaccggc cagccatgaa ttacgacaag ctgagccgct
     1681 cgctccgata ctattatgag aaaggcatca tgcagaaggt ggctggtgag cgttacgtgt
     1741 acaagtttgt gtgtgagccc gaggccctct tctctttggc cttcccggac aatcagcgtc
     1801 cagctctcaa ggctgagttt gaccggcctg tcagtgagga ggacacagtc cctttgtccc
     1861 acttggatga gagccccgcc tacctcccag agctggctgg ccccgcccag ccatttggcc
     1921 ccaagggtgg ctactcttac tacccaactt tcttgtacaa agtggttgat atcggtaagc
     1981 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt accggttaag
     2041 tcgacaatca acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     2101 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     2161 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     2221 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     2281 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     2341 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     2401 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     2461 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     2521 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     2581 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     2641 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     2701 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     2761 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     2821 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     2881 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     2941 gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta
     3001 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     3061 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3121 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     3181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     3301 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     3361 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3421 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3481 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     3541 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3601 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3661 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3721 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     3781 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     3841 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     3901 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     3961 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     4021 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4081 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4141 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4201 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4261 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4321 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4381 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4441 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4501 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4561 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4621 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4681 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4741 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4801 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4861 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4921 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4981 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5041 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5101 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5161 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5221 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5281 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5341 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5401 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5461 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5521 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5581 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5641 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5701 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5761 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5821 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5881 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     5941 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     6001 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     6061 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     6121 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     6181 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     6241 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     6301 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     6361 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     6421 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     6481 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6541 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6601 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     6661 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     6721 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     6781 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     6841 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     6901 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     6961 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     7021 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     7081 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     7141 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     7201 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     7261 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     7321 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     7381 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     7441 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     7501 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     7561 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     7621 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     7681 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     7741 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     7801 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     7861 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     7921 ccatgcattg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     7981 aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac
     8041 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8101 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8161 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccat
     8221 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
     8281 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg
     8341 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt
     8401 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
     8461 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca
     8521 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg
     8581 tgaattgctg ccctctggtt atgtgtggga gggcctgcag ctgcagtagt aaggcgcgcc
     8641 gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
     8701 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     8761 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     8821 gacagcagag atccactttg gcgcc
//
