LOCUS       sequence-142600-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PAHA
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             457..474
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(527..545)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      541..588
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(712..731)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gggacaggaa acaagctata ttgaagacaa
      121 ctgcaatcaa aatggtgcca tatcgctgat cttctcactc aaagaagaag ttggtgcatt
      181 ggccaaagta ttgcgcttat ttgaggagaa tgatgtaaac ctgacccaca ttgaatctag
      241 accttctcgt ttaaagaaag atgagtatga atttttcacc catttggata aacgtagcct
      301 gcctgctctg acaaacatca tcaagatctt gaggcatgac attggtgcca ctgtccatga
      361 gctttcacga gataagaaga aagacacagt gtgacagtaa aggtggatac ggatccgaat
      421 tcgagctccg tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat
      481 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      541 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      601 actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      661 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      721 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      781 ggctccctt
//
