LOCUS       sequence-142601-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PAHA
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(407..427)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(452..469)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             480..502
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    517..541
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(541..560)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(542..560)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(644..663)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atccgtatcc acctttactg tcacactgtg tctttcttct
      121 tatctcgtga aagctcatgg acagtggcac caatgtcatg cctcaagatc ttgatgatgt
      181 ttgtcagagc aggcaggcta cgtttatcca aatgggtgaa aaattcatac tcatctttct
      241 ttaaacgaga aggtctagat tcaatgtggg tcaggtttac atcattctcc tcaaataagc
      301 gcaatacttt ggccaatgca ccaacttctt ctttgagtga gaagatcagc gatatggcac
      361 cattttgatt gcagttgtct tcaatatagc ttgtttcctg tcccatggat tggaagtaca
      421 ggttctcggt acccagatct acaccagaag aatgatgatg atgatggtgc atatgtatat
      481 ctccttctta aagttaaaca aaattatttc tagaggggaa ttgttatccg ctcacaattc
      541 ccctatagtg agtcgtatta atttcgcggg atcgagatct cgatcctcta cgccggacgc
      601 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
      661 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
      721 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
      781 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct a
//
