LOCUS       sequence-142604-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PHIPA
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..25)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(472..492)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(517..534)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             545..567
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    582..606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(606..625)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(607..625)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(709..728)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gatctcagtg gtggtggtgg tggtgctcga gtgcggccgc aagcttgtcg acggagctcg
       61 aattcggatc cgtatccacc tttactgtca gcgtttggta atggtattac gtttatgaaa
      121 gcgcagcgcg cttttataat cgctcagcac gctgctaatg tgttcttcaa aaaacgcgct
      181 cagacgcagg ctcatgctat agatacggct acgtttgctc ggggtatagg ctttgctgtt
      241 gctaaaaatc aggcgcacat ctttgcacag ttccatcggg ctttcatagt tacccgcttc
      301 cagggtttcg cgcacggtcg caaaatccat cggggtatca atgatatcgc gataatccgg
      361 atattccagc agatcaaccg gctgacgaaa cggttcgcta tcttcacact gaaaaatcag
      421 attcagcagt tcttcgcact gttttttcca cgcctggata tcatagctca tggattggaa
      481 gtacaggttc tcggtaccca gatctacacc agaagaatga tgatgatgat ggtgcatatg
      541 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
      601 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg
      661 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
      721 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
      781 tgggtatggt ggcaggcccc gtggccgggg gactggttgg gcgc
//
