LOCUS       sequence-142605-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PHIPA
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..22
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             514..531
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(584..602)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      598..645
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tatgcaccat catcatcatc attcttctgg tgtagatctg ggtaccgaga acctgtactt
       61 ccaatccatg agctatgata tccaggcgtg gaaaaaacag tgcgaagaac tgctgaatct
      121 gatttttcag tgtgaagata gcgaaccgtt tcgtcagccg gttgatctgc tggaatatcc
      181 ggattatcgc gatatcattg ataccccgat ggattttgcg accgtgcgcg aaaccctgga
      241 agcgggtaac tatgaaagcc cgatggaact gtgcaaagat gtgcgcctga tttttagcaa
      301 cagcaaagcc tataccccga gcaaacgtag ccgtatctat agcatgagcc tgcgtctgag
      361 cgcgtttttt gaagaacaca ttagcagcgt gctgagcgat tataaaagcg cgctgcgctt
      421 tcataaacgt aataccatta ccaaacgctg acagtaaagg tggatacgga tccgaattcg
      481 agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg
      541 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      601 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      661 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      721 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      781 tcttcc
//
