LOCUS       sequence-142607-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BRD1A
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(479..499)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(524..541)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             552..574
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    589..613
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(613..632)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(614..632)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(716..735)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg
       61 gagctcgaat tcggatccgt atccaccttt actgtcaaga aggccgctca ggcaggtgca
      121 tccccgaggc ctcttccaag ccgatgctgt ccacctcgcg ccgggcctgc ctcagaacaa
      181 cacctccctg atcgcgcagc ctcaccgcgg ctctatagaa cacggtgtcc ctggcattgt
      241 acttcatgca gttatctata atgagatcaa aatcctcctc aaactcatgg aggtttttat
      301 acccttgagc ttctaaccgt ttcctcattg tggcaaagtc catgggatgt ttaatgtgat
      361 ccaaataatc tggtacctcc ttcagactca cgggctgcgc aaatatcctg gcggggtcct
      421 tgtcttgcag ctggtccagc actgagcgca gcagcaccgt cagcggggtc agccgcatgg
      481 attggaagta caggttctcg gtacccagat ctacaccaga agaatgatga tgatgatggt
      541 gcatatgtat atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc
      601 cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc
      661 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
      721 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgct
//
