LOCUS       sequence-142613-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AASDHPPT
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tttaataagg agatatacca tgggcagcag ccatcatcat catcatcacc acagcagcgg
       61 cagagaaaac ttgtatttcc agggccatat ggagggcgtg cgctgggcct tttcctgcgg
      121 cacttggctg ccgagccgag ccgaatggct gctggcagtg cgatcgattc agcccgagga
      181 gaaggagcgc attggccagt tcgtctttgc ccgggacgct aaggcagcca tggctggtcg
      241 tctgatgata aggaaattag ttgcagagaa attgaatatc ccttggaatc atattcgttt
      301 gcaaagaact gcaaaaggaa aaccagttct tgcaaaggac tcatcgaatc cttacccgaa
      361 tttcaacttt aacatctctc atcaaggaga ctatgcagtg cttgctgctg aacctgagct
      421 gcaagttgga attgatataa tgaagactag ttttccaggt cgtggttcaa ttccagaatt
      481 ctttcatatt atgaaaagaa agtttaccaa caaagaatgg gaaacaatca gaagctttaa
      541 ggatgagtgg actcagctgg atatgtttta taggaattgg gcacttaagg aaagcttcat
      601 aaaagccatt ggtgttggac taggatttga attgcagcgg cttgaatttg atctatctcc
      661 attaaacttg gatataggcc aagtttataa agaaacacgt ttattcctgg atggagagga
      721 agaaaaagaa tgggcatttg aggaaagcaa aatagatgag caccattttg ttgcagttgc
      781 tcttaggaaa cccgatggat ctagacatca ggatgttc
//
