LOCUS       sequence-142636-         323 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Monitor post-translational degradation of Aiolos AA130-189 
            (Lentiviral, 
            SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.Pur
            oR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 323)
  TITLE     Artichoke
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Aiolos AA130-189 in Artichoke
FEATURES             Location/Qualifiers
     source          1..323
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(232..250)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
       61 tggccacaac cctggaattc gccaccatgg tgagcaaggg cgaggaggat aacatggcca
      121 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
      181 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      241 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
      301 acggctccaa ggcctacgtg aag
//
